The enhanced chemotherapeutic effects of doxorubicin loaded PEG coated TiO2 nanocarriers in an orthotopic breast tumor bearing mouse model
The enhanced chemotherapeutic effects of doxorubicin loaded PEG coated TiO2 nanocarriers in an orthotopic breast tumor bearing mouse model
复制标题
负载阿霉素的 PEG 包被的 TiO2 纳米载体在原位乳腺肿瘤小鼠模型中增强化疗效果
DOI：
10.1039/c4tb01781a
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发表时间：
2015-01-01
影响因子：
7
通讯作者：
Tian, Jie
中科院分区：
文献类型：
--
作者：
Du, Yang;Ren, Wenzhi;Tian, Jie
许多用于癌症治疗的化疗药物在体内递送至肿瘤期间会遇到问题，并且具有高水平的全身毒性。提高药物输送效率最显着的进展之一是探索各种类型的纳米颗粒（NP）作为药物载体。最近的研究表明，二氧化钛（TiO2）纳米载体即使在体外对多重耐药癌症也具有药物输送和治疗的潜力。此外，体外实验证明，通过负载TiO2纳米粒子，可以增强阿霉素（DOX）在乳腺癌细胞中的抗癌活性。然而，目前还没有体内动物模型的证据，这是其进一步临床应用的关键一步。本研究的目的是探索新型 TiO2-PEG-DOX 纳米颗粒，即负载 DOX 的聚乙二醇 (PEG) 包覆的 TiO2 纳米载体，并研究其在原位乳腺肿瘤小鼠中控制药物释放和增强 DOX 化疗效果的潜在应用。通过生物发光成像（BLI）动态监测肿瘤生长和药物治疗效果，并评估纳米粒子在体内使用的安全性。研究发现，与游离 DOX 治疗相比，TiO2-PEG-DOX 纳米颗粒具有更高的抗肿瘤功效，且没有明显的副作用。我们的研究表明，PEG 涂层的 TiO2 纳米载体是一种安全且有潜力的平台，可用于有效的药物输送并最大限度地减少化疗药物的全身毒性。首次证明TiO2基纳米载体可增强阿霉素体内化疗效果。
Many chemotherapeutics used for cancer treatments encounter issues during delivery to tumors in vivo and have high levels of systemic toxicity. One of the most prominent progresses in improving drug delivery efficiency is through exploring various types of nanoparticles (NPs) as drug carriers. Recent studies have demonstrated that titanium dioxide (TiO2) nanocarriers have potential for drug delivery and therapy even in multidrug resistant cancers in vitro. Moreover, it was proved that the anticancer activity of doxorubicin (DOX) was enhanced by loading onto TiO2 nanoparticles in breast cancer cells in vitro. However, there is no evidence from the animal model in vivo, which is a critical step for their further clinical applications. The aim of this study was to explore novel TiO2-PEG-DOX nanoparticles, the DOX loaded polyethylene glycol (PEG) coated TiO2 nanocarriers, and investigate their potential application in enabling controlled drug release and enhancing the chemotherapeutic efficacy of DOX in the orthotopic breast tumor bearing mice. The tumor growth and drug treatment efficacy were dynamically monitored by bioluminescence imaging (BLI), and the safety of NPs for in vivo usage was also evaluated. It was found that TiO2-PEG-DOX nanoparticles possessed improved antitumor efficacy without observable side effects compared to the free DOX treatment. Our study suggested that the PEG coated TiO2 nanocarrier is a safe and potential platform for the efficient drug delivery and minimizing the systemic toxicity of chemotherapeutic agents. It has been proved for the first time that TiO2-based nanocarriers enhance the chemotherapeutic effects of doxorubicin in vivo.