VARIANT FORMS OF MITOCHONDRIAL TRANSLATION PRODUCTS IN YEAST - EVIDENCE FOR LOCATION OF DETERMINANTS ON MITOCHONDRIAL-DNA
VARIANT FORMS OF MITOCHONDRIAL TRANSLATION PRODUCTS IN YEAST - EVIDENCE FOR LOCATION OF DETERMINANTS ON MITOCHONDRIAL-DNA
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DOI：
10.1073/pnas.73.4.1083
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
BUTOW, RA
中科院分区：
文献类型：
--
作者：
DOUGLAS, MG;BUTOW, RA
用~（35）SO_（42）~-标记酵母线粒体蛋白质合成产物。通过十二烷基硫酸钠-指数聚丙烯酰胺平板凝胶分析，已检测到20多种符合线粒体翻译产物标准的多肽。在两个野生型菌株的线粒体翻译产物的比较，揭示了一些多肽物种的变异形式，显示其结构基因在mtDNA上的位置一致的遗传行为。我们的研究结果表明，通过直接检测特定多肽种类的分离和重组行为，对野生型酵母中mtDNA的推定基因产物进行遗传分析是可行的。
Products of mitochondrial protein synthesis in yeast have been labeled in vivo with 35SO42-. More than 20 polypeptide species fulfilling the criteria of mitochondrial translation products have been detected by analysis on sodium dodecyl sulfate-exponential polyacrylamide slab gels. A comparison of mitochondrial translation products in two wild-type strains has revealed variant forms of some polypeptide species which show genetic behavior consistent with the location of their structural genes on mtDNA. Our results demonstrate the feasibility of performing genetic analysis on putative gene products of mtDNA in wild-type yeast by direct examination of the segregation and recombination behavior of specific polypeptide species.