Monitoring of wound healing process of human skin after fractional laser treatments with optical coherence tomography.
Monitoring of wound healing process of human skin after fractional laser treatments with optical coherence tomography.
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DOI：
10.1364/boe.4.002362
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发表时间：
2013-11
影响因子：
3.4
通讯作者：
M. Tsai;Chih-Hsun Yang;S. Shen;Ya-Ju Lee;F. Chang;Cheng-Shin Feng
中科院分区：
文献类型：
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作者：
M. Tsai;Chih-Hsun Yang;S. Shen;Ya-Ju Lee;F. Chang;Cheng-Shin Feng
由非烧蚀分数激光（nafl）或烧蚀分数激光（afl）诱导的分数光热分解可以重塑皮肤，再生胶原蛋白，并去除肿瘤组织。然而，分次激光治疗可能会导致严重的副作用，需要多次治疗才能达到预期的效果。因此，分步激光治疗后的治疗结果和停药时间是决定后续治疗策略的关键问题。在这项研究中，光学相干断层扫描（OCT）系统被用于NAFL和AFL治疗后伤口愈合的体内研究。根据OCT扫描结果，可以从形态学上确定激光诱导的光热分解包括挥发和凝固。为了继续监测创面愈合过程，在不同时间点对治疗区域进行OCT扫描，并从三维OCT图像中提取不同组织深度的正面图像。此外，为了定量评估伤口愈合过程中不同组织深度的形态学变化，开发了一种算法来区分未处理和处理组织的后向散射特性。结果表明，nafl引起的凝血损伤可在6 d内迅速愈合。相比之下，afl诱导的组织挥发需要更长的恢复时间，为14天。总之，本研究建立了该方法作为临床监测治疗结果和局部激光治疗后伤口愈合的一种手段的可行性。
Fractional photothermolysis induced by non-ablative fractional lasers (NAFLs) or ablative fractional lasers (AFLs) can remodel the skin, regenerate collagen, and remove tumor tissue. However, fractional laser treatments may result in severe side effects, and multiple treatments are required to achieve the expected outcome. Thus, the treatment outcome and downtime after fractional laser treatments are key issues to determine the following treatment strategy. In this study, an optical coherence tomography (OCT) system was implemented for in vivo studies of wound healing after NAFL and AFL treatments. According to the OCT scanning results, the laser-induced photothermolysis including volatilization and coagulation could be morphologically identified. To continue monitoring the wound healing process, the treated regions were scanned with OCT at different time points, and the en-face images at various tissue depths were extracted from three-dimensional OCT images. Furthermore, to quantitatively evaluate the morphological changes at different tissue depths during wound healing, an algorithm was developed to distinguish the backscattering properties of untreated and treated tissues. The results showed that the coagulation damage induced by the NAFLs could be rapidly healed in 6 days. In contrast, the tissue volatilization induced by AFLs required a longer recovery time of 14 days. In conclusion, this study establishes the feasibility of this methodology as a means of clinically monitoring treatment outcomes and wound healing after fractional laser treatments.