Suppression of the biosynthesis of cellular sphingolipids results in the inhibition of the maturation of influenza virus particles in MDCK cells
Suppression of the biosynthesis of cellular sphingolipids results in the inhibition of the maturation of influenza virus particles in MDCK cells
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DOI：
10.1248/bpb.29.1575
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发表时间：
2006-08-01
影响因子：
2
通讯作者：
Suzuki, Takashi
中科院分区：
文献类型：
--
作者：
Hidari, Kazuya I. P. J.;Suzuki, Yasuo;Suzuki, Takashi
为了研究细胞鞘糖脂参与宿主细胞中流感病毒的繁殖，用鞘糖脂生物合成抑制剂伏马菌素B1和d，1-苏型-1-苯基-2-癸酰氨基-3-吗啉代-1-丙醇处理MDCK细胞。在病毒接种前后用任一抑制剂连续处理细胞，但单独预处理不能显著降低病毒感染，但不能降低病毒对细胞的附着。免疫细胞化学分析表明，细胞分布的血凝素，病毒糖蛋白，被彻底改变时，细胞连续处理的抑制剂在前和后病毒接种，但不是单独的预处理。我们的研究结果有力地表明，细胞鞘脂在病毒吸附到宿主细胞后的事件中发挥重要作用。
To investigate involvement of cellular glycosphingolipids in the propagation of influenza viruses in host cells, MDCK cells were treated with inhibitors for sphingolipid biosynthesis, fumonisin B1 and d,1-threo-1-phenyl-2decanoylamino-3-morpholino-1-propanol. Continuous treatment of the cells with either inhibitor during pre- and post viral inoculation, but not the pretreatment alone, significantly reduced viral infection, but not viral attachment to the cells. Immunocytochemical analysis demonstrated that cellular distribution of hemagglutinin, a viral glycoprotein, was drastically altered when the cells were continuously treated with the inhibitors during pre- and post viral inoculation, but not the pretreatment alone. Our findings strongly suggest that cellular sphingolipids play important roles in the events after viral adsorption to the host cells.