Functional characteristics of Svl3 and Pam1 that are required for proper cell wall formation in yeast cells
Functional characteristics of Svl3 and Pam1 that are required for proper cell wall formation in yeast cells
复制标题
酵母细胞中正确形成细胞壁所需的 Svl3 和 Pam1 功能特征
DOI：
10.1002/yea.3502
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发表时间：
2020
期刊：
影响因子：
2.6
通讯作者：
Nakanishi Hideki
中科院分区：
文献类型：
--
作者：
Jin Yifan;Okamoto Michiyo;Chibana Hiroji;Liu Guoyu;Gao Xiao-Dong;Nakanishi Hideki
在芽殖酵母Saccharomyces cerevisiae中，Svl 3和Pam 1蛋白作为功能同源物起作用。其功能的丧失导致细胞壁中几丁质沉积水平增加和温度敏感性增加，表明它们参与细胞壁形成。我们发现这些蛋白质的N-和C-末端具有独特的关键功能。它们含有一个可能具有2-脱氢泛解酸2-还原酶结构域的N-末端部分。在Svl 3中，该部分可以被酵母2-脱氢泛解酸2-还原酶Pan 5替代，表明Svl 3及其同源物可能能够介导2-脱氢泛解酸2-还原酶功能。另一方面，Svl 3通过C末端部分中的多个定位信号被募集到芽尖和芽颈。这些信号之一是位于C末端的富含赖氨酸的区域。Svl 3的功能和定位被这个富含赖氨酸的区域的丢失显著破坏;然而，其定位并没有被突变完全消除，因为另一个定位信号能够实现适当的转运。Svl 3和Pam 1直向同源物存在于真菌物种的细胞中。光滑假丝酵母的Svl 3直向同源物可以补充S. gallae.C.中Svl 3和Pam 1的缺失。缺失SVL 3和PAM 1直向同源基因的光滑细胞表现出与在vl 3中观察到的相似的表型。党卫队因此，Svl 3同源物通常可能是真菌细胞中细胞壁组装所需的。
In the budding yeastSaccharomyces cerevisiae, Svl3 and Pam1 proteins work as functional homologues. Loss of their function causes increased levels of chitin deposition in the cell wall and temperature sensitivity, suggesting their involvement in cell wall formation. We found that the N‐ and C‐termini of these proteins have distinctive and critical functions. They contain an N‐terminal part that has a probable 2‐dehydropantoate 2‐reductase domain. In Svl3, this part can be replaced with the yeast 2‐dehydropantoate 2‐reductase, Pan5, suggesting that Svl3 and its homologues may be able to mediate 2‐dehydropantoate 2‐reductase function. On the other hand, Svl3 is recruited to the bud tip and bud neck via multiple localization signals in the C‐terminal part. One of such signals is the lysine‐rich region located in the C‐terminal end. The function and localization of Svl3 are significantly disrupted by the loss of this lysine‐rich region; however, its localization is not completely abolished by the mutation because another localization signal enables appropriate transport. Svl3 and Pam1 orthologues are found in cells across fungal species. The Svl3 orthologues ofCandida glabratacan complement the loss of Svl3 and Pam1 inS. cerevisiae.C. glabratacells lacking theSVL3andPAM1orthologue genes exhibit phenotypes similar to those observed insvl3∆pam1∆ S. cerevisiaecells. Thus, Svl3 homologues may be generally required for the assembly of the cell wall in fungal cells.