Enlargement of calcium oxalate stones to clinically significant size in an in-vitro stone generator
Enlargement of calcium oxalate stones to clinically significant size in an in-vitro stone generator
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DOI：
10.1046/j.1464-410x.2002.03027.x
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发表时间：
2002-12-01
影响因子：
4.5
通讯作者：
Rao, PN
中科院分区：
文献类型：
--
作者：
Ananth, K;Kavanagh, JP;Rao, PN
目的建立并验证一种适用于草酸钙结石生长的体外定量研究的方法，使草酸钙结石生长到临床上有意义的大小。材料和方法将草酸钙结石的小碎片悬浮在混合悬浮液/混合产物去除结晶器中，该结晶器提供草酸钙过饱和的人工尿液。定期对碎片进行称重，直至达到约500毫克。将结果绘制为重量对时间，并拟合到对应于直径恒定增加、表面积控制和恒定沉积生长模式的方程。结果在137 ~ 369 h内，8个2-6 mm的碎片生长到10 mm。7条生长曲线通过方程w = kt（（312））+c最佳拟合（r（2）大于或等于0.988），其中w是重量，k是生长常数，t是时间，c是近似于初始重量的常数。这对应于一个表面积依赖mechanism.Conclusions的增长，这些小片段的临床显着的大小加速整个实验期间的方式是一致的表面积依赖的机制。我们已经开发了一个弹性模型，适用于研究草酸钙结石生长的动力学在体外。
Objective To develop and validate an in vitro method suitable for the quantitative investigation of the growth of calcium oxalate stones through to a clinically significant size.Materials and methods Small fragments of calcium oxalate calculi were suspended in a mixed suspension/mixed product removal crystalliser supplied with artificial urine supersaturated with calcium oxalate. The fragments were weighed at regular intervals until they reached approximate to 500 mg. The results were plotted as weight against time and fitted to equations corresponding to constant increase in diameter, surface area-controlled and constant-deposition growth patterns. The choice of the most appropriate model was based on the squared regression coefficient (r(2)).Results Eight fragments (2-6 mm in diameter) were grown to 10 mm in diameter over periods from 137 to 369 h. Seven of the growth curves were best-fitted (r(2) greater than or equal to 0.988) by the equation w = kt((312)) +c, where w is the weight, k is a growth constant, t is the time and c is a constant approximating to the initial weight. This corresponds to a surface area-dependent mechanism.Conclusions The growth of these small fragments to a clinically significant size accelerated throughout the experimental period in a way which was consistent with a surface area-dependent mechanism. We have developed a resilient model suitable for studying the kinetics of calcium oxalate stone growth in vitro.