Quantitative isolation of Verticillium spp. from soil and moribund potato haulm
Quantitative isolation of Verticillium spp. from soil and moribund potato haulm
复制标题
黄萎病菌的定量分离。
DOI：
10.1111/j.1744-7348.1971.tb02918.x
复制
发表时间：
1971
期刊：
影响因子：
--
通讯作者：
J. Harrison
中科院分区：
文献类型：
--
作者：
I. Isaac;P. Fletcher;J. Harrison
总结
一种高比例分离重要轮枝菌繁殖体的技术。土壤中的微菌核和分生孢子组分的定量区分以及干马铃薯茎中微菌核群体及其生活力的估计。
在盆栽条件下，土壤中的大丽轮枝菌种群数量低于c. 40个繁殖体/g不能诱导马铃薯植株发病;随着群体的增大，病害严重程度与真菌群体之间存在一定的关系。在人工感染大丽轮枝菌（V. dahliae）的土壤中，50000个繁殖体/g干土），存活种群在6个月内下降至C. 100个繁殖体/g，然后保持稳定超过一年。从南威尔士和东英吉利的田野中获得了大种群的V. nigrescens和V. lateritium以及小种群的V. dahliae。
SUMMARY
A technique to isolate a high proportion of the propagules of important Verticillium spp. from soils is described with modifications for quantitatively distinguishing between the microsclerotial and conidial fractions of V. dahliae and estimating the microsclerotial population and its viability in dried potato stems.
In pots, soil populations of V. dahliae with less than c. 40 propagules/g failed to induce disease in potato plants; with larger populations there was some relation between disease severity and fungal population. In soils artificially infested with V. dahliae (c. 50000 propagules/g dried soil) the viable population decreased within 6 months to c. 100 propagules/g and then remained stable for more than a year. From fields in South Wales and East Anglia large populations of V. nigrescens and V. lateritium and small populations of V. dahliae were obtained.