Aeromonas australiensis sp nov., isolated from irrigation water
Aeromonas australiensis sp nov., isolated from irrigation water
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DOI：
10.1099/ijs.0.040162-0
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发表时间：
2013-06-01
影响因子：
2.8
通讯作者：
Jose Figueras, Maria
中科院分区：
文献类型：
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作者：
Aravena-Roman, Max;Beaz-Hidalgo, Roxana;Jose Figueras, Maria
从西澳大利亚西南部的灌溉水系统中分离出一种革兰氏阴性兼性厌氧杆菌，命名为菌株 266(T)。 16S rRNA基因序列分析证实，菌株266（T）属于气单胞菌属，最接近的物种是河流气单胞菌（与模式菌株相似性为99.6％，有6个核苷酸差异），其次是维罗尼气单胞菌和嗜糖气单胞菌（均为99.5％）。 gyrB 和 rpoD 序列分析表明，菌株 266(T) 形成了独立于该属其他物种的系统发育系。使用六个管家基因（gyrB、rpoD、recA、dnaJ、gyrA 和 dnaX）的串联序列证实了这一点，这也表明 A. veronii 和 A. allosaccharophila 是最近的亲戚。 DNA-DNA重关联实验和表型分析进一步支持菌株266(T)代表新物种的结论，其名称为Aeromonas australiensis sp。十一月建议采用应变类型 266(T) (=CECT 8023(T) =LMG 2670(T))。
A Gram-negative, facultatively anaerobic bacillus, designated strain 266(T), was isolated from an irrigation water system in the south-west of Western Australia. Analysis of the 16S rRNA gene sequence confirmed that strain 266(T) belonged to the genus Aeromonas, with the nearest species being Aeromonas fluvialis (99.6 % similarity to the type strain, with 6 nucleotide differences) followed by Aeromonas veronii and Aeromonas allosaccharophila (both 99.5 %). Analysis of gyrB and rpoD sequences suggested that strain 266(T) formed a phylogenetic line independent of other species in the genus. This was confirmed using the concatenated sequences of six housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA and dnaX) that also indicated that A. veronii and A. allosaccharophila were the nearest relatives. DNA-DNA reassociation experiments and phenotypic analysis further supported the conclusion that strain 266(T) represents a novel species, for which the name Aeromonas australiensis sp. nov. is proposed, with type strain 266(T) (=CECT 8023(T) =LMG 2670(T)).