LIGHT MICROSCOPIC CHARACTERIZATION OF THE FIBROBLAST-LIKE SYNOVIAL INTIMAL CELL (SYNOVIOCYTE)
LIGHT MICROSCOPIC CHARACTERIZATION OF THE FIBROBLAST-LIKE SYNOVIAL INTIMAL CELL (SYNOVIOCYTE)
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DOI：
10.1002/art.1780351010
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发表时间：
1992-10-01
影响因子：
--
通讯作者：
EDWARDS, JCW
中科院分区：
文献类型：
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作者：
WILKINSON, LS;PITSILLIDES, AA;EDWARDS, JCW
客观的。通过光学显微镜重新评估滑膜内膜细胞群。方法。通过一系列组合的免疫组织化学和细胞化学染色技术，以组织切片和细胞离心涂片制备物的形式对非炎症、类风湿和骨关节炎滑液进行分析。结果。鉴定出两个内膜细胞群。第一个携带巨噬细胞标记。第二个显示高尿苷二磷酸葡萄糖脱氢酶 (UDPGD) 活性、最小的细胞质 CD68、缺乏非特异性酯酶 (NSE) 活性以及缺乏白细胞和内皮抗原。这些细胞中的大多数表现出高含量的脯氨酰羟化酶。结论。 NSE 和 UDPGD 活性的组合细胞化学染色可以有效分离内膜细胞群。我们认为具有高UDPGD活性的细胞是电镜下定义的成纤维细胞样或B型滑膜内膜细胞。高 UDPGD 活性可能反映了优先合成糖胺聚糖（包括透明质酸）的能力。
Objective. To reassess synovial intimal cell populations by light microscopy.Methods. Non-inflamed, rheumatoid and osteoarthritic synovia were analyzed as tissue sections and cytospin preparations by a series of combined immunohistochemical and cytochemical staining techniques.Results. Two populations of intimal cells were identified. The first carried macrophage markers. The second showed high uridine diphosphoglucose dehydrogenase (UDPGD) activity, minimal cytoplasmic CD68, absent non-specific esterase (NSE) activity, and absent leukocyte and endothelial antigens. The majority of these cells showed a high content of prolyl hydroxylase.Conclusion. Combined cytochemical staining for NSE and UDPGD activity allows effective separation of intimal cell populations. We suggest that the cells of high UDPGD activity are the fibroblast-like or type B synovial intimal cells defined by electron microscopy. High UDPGD activity probably reflects a preferential ability to synthesize glycosaminoglycans, including hyaluronan.