Zika virus infection damages the testes in mice.
Zika virus infection damages the testes in mice.
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DOI：
10.1038/nature20556
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发表时间：
2016-12-15
期刊：
影响因子：
64.8
通讯作者：
Diamond MS
中科院分区：
文献类型：
--
作者：
Govero J;Esakky P;Scheaffer SM;Fernandez E;Drury A;Platt DJ;Gorman MJ;Richner JM;Caine EA;Salazar V;Moley KH;Diamond MS
孕妇感染寨卡病毒（ZIKV）可导致包括小头畸形在内的先天性畸形，这引起了全球对这种新兴病原体的关注。除了蚊子传播外，ZIKV还可以在受影响男性的精液中检测到很长一段时间，并通过性传播。在这里，使用小鼠适应的非洲ZIKV毒株（Dakar 41519），我们评估了小鼠雄性生殖道中感染的后果。我们在雄性小鼠的睾丸和附睾中观察到ZIKV的持续存在，而不是密切相关的登革病毒（DENV），这与导致睾酮和白蛋白B水平降低的组织损伤以及少精子症有关。ZIKV优先感染睾丸中的精原细胞、初级精母细胞和支持细胞，导致细胞死亡和曲细精管的破坏。用当代亚洲ZIKV毒株（H/PF/2013）观察到的损伤较小，部分原因是该病毒在小鼠中复制效率较低。小鼠中的这些观察结果在多大程度上转化为人类尚不清楚，但ZIKV感染者精子功能和活力的纵向研究似乎是有必要的。
Zika virus (ZIKV) infection of pregnant women can cause congenital malformations including microcephaly, which has focused global attention on this emerging pathogen. In addition to transmission by mosquitoes, ZIKV can be detected in the seminal fluid of affected males for extended periods of time and transmitted sexually. Here, using a mouse-adapted African ZIKV strain (Dakar 41519) we evaluated the consequences of infection in the male reproductive tract of mice. We observed persistence of ZIKV, but not the closely related Dengue virus (DENV), in the testis and epididymis of male mice, and this was associated with tissue injury that caused diminished testosterone and inhibin B levels, and oligospermia. ZIKV preferentially infected spermatogonia, primary spermatocytes, and Sertoli cells in the testis, resulting in cell death and destruction of the seminiferous tubules. Less damage was observed with a contemporary Asian ZIKV strain (H/PF/2013), in part because this virus replicates less efficiently in mice. The extent to which these observations in mice translate to humans remains unclear, but longitudinal studies of sperm function and viability in ZIKV-infected humans seem warranted.