Expression and evolutionary analysis of soluble inorganic pyrophosphatase gene family in pear and four other Rosaceae species
Expression and evolutionary analysis of soluble inorganic pyrophosphatase gene family in pear and four other Rosaceae species
复制标题
梨等四种蔷薇科植物可溶性无机焦磷酸酶基因家族的表达及进化分析
DOI：
10.1007/s00606-020-01628-0
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发表时间：
2020
影响因子：
1.9
通讯作者：
Shaoling Zhang
中科院分区：
文献类型：
--
作者：
Chao Tang;Xin Qiao;Xiaoxuan Zhu;Waqar Khan;Juyou Wu;Shaoling Zhang
在植物中，可溶性无机焦磷酸酶（PPA）在无机焦磷酸盐水解中起着不可或缺的作用。然而，PPA基因家族在梨和蔷薇科植物中的表达和进化历史尚不清楚。本研究从梨（Pyrus bretschneideri）、苹果（Malus arteritica）、桃（Prunus persica）、草莓（Fragaria vesca）和梅（Prunus mume）等5种蔷薇科植物中克隆了39个PPA基因。根据基因的结构特征和系统发育分析，将PPA基因家族成员分为两个主要亚家族（A和B）。全基因组复制和分散基因复制是PPA基因家族在梨等蔷薇科植物中扩展的主要动力。纯化选择在PPA基因的进化中起主导作用。亚细胞定位分析表明，梨PPA基因定位于细胞质、叶绿体或核膜附近，在梨不同组织中表现出多种表达模式。PPA基因家族中有几个成员仅在一种组织中表达量较高，如PbrPPA 2、PbrPPA 5和PbrPPA 8。表达分析表明，5个PPA基因参与了梨花粉管的生长，其中4个PPA基因对过量的PPi有反应。综上所述，本研究建立了梨和蔷薇科植物PPA基因家族的功能特征和进化图谱，为进一步研究PPA基因在梨花粉管生长中的功能提供了基因表达数据。
In plants, soluble inorganic pyrophosphatase (PPA) play an indispensable role in inorganic pyrophosphate hydrolysis. However, the expression and the evolutionary history ofPPAgene family remain unclear in pear and Rosaceae species. In this study, thirty-ninePPAgenes were identified from five Rosaceae species including pear (Pyrus bretschneideri), apple (Malus domestica), peach (Prunus persica), strawberry (Fragaria vesca) and Japanese apricot/mei (Prunus mume). Based on the structural characteristics of the genes and phylogenetic analysis, members ofPPAgene family were classified into two main subfamilies (A and B). Whole-genome duplication (WGD) and dispersed gene duplication were the main force, which drove the expansion ofPPAgene family in pear and other Rosaceae species. Purifying selection played a dominant role in the evolution ofPPAgenes. Subcellular localization analysis demonstrated that pearPPAgenes located in the cytosol, chloroplasts or near the nuclear envelope.PPAgenes showed multiple expression patterns in different pear tissues. Several members ofPPAgene family presented particularly higher expression level in only one of the six tissues, such asPbrPPA2,PbrPPA5andPbrPPA8. Expression analysis showed that fivePPAgenes were involved in the growth of pear pollen tube, meanwhile fourPPAgenes could respond to excess of PPi in pear pollen tube. Overall, we established a comprehensive landscape in the functional characterization and evolution ofPPAgene family in pear and Rosaceae species, and provided gene expression data for exploring the functional roles ofPPAgenes in the growth of pear pollen tube.