A factor in spent media from Sertoli cell-enriched cultures that stimulates steroidogenesis in Leydig cells
A factor in spent media from Sertoli cell-enriched cultures that stimulates steroidogenesis in Leydig cells
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来自支持细胞富集培养物的废培养基中的一种因子，可刺激 Leydig 细胞中的类固醇生成
DOI：
10.1016/0303-7207(85)90158-3
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发表时间：
1985
影响因子：
4.1
通讯作者：
J. Ćailleau
中科院分区：
文献类型：
--
作者：
G. Verhoeven;J. Ćailleau
我们研究了支持细胞培养上清液(SCM)中是否含有影响间质细胞类固醇生成的一个或多个因子。新鲜制备的青春期前间质细胞或Percoll纯化的间质细胞以及在有或无黄体生成素存在或不存在的情况下培养的间质细胞和类似细胞，在5α还原酶抑制剂存在和存在或不存在干细胞的情况下孵育24 h。放射免疫法测定19-类固醇(睾酮和雄烯二酮)、C21-类固醇(孕酮、17-α-羟孕酮和20-α-羟孕酮-4-烯-3-酮)和环磷酸腺苷的蓄积。可以证明，SCM含有一种刺激类固醇合成途径早期步骤的因子，但同时也阻碍了C21前体向雄激素的转化。在新鲜制备的间质细胞中，最终的效果是刺激雄激素的输出。在没有黄体生成素的情况下培养的间质细胞，主要是C21-类固醇产量增加。这些生物学效应与LHRH及其激动剂所观察到的相似。然而，支持细胞因子的活性不受LHRH拮抗剂的影响，并且该因子和LHRH的最大有效浓度具有相加效应，表明它们通过不同的受体系统发挥作用。初步表征表明，SCM中的因子是一种分子量为10000的不耐热蛋白质。在无血清培养基长时间培养过程中，该因子的产生减少。添加胎牛；小牛血清可显著增加该因子的产生或活性，且呈剂量依赖关系。几种永久性细胞系(B16、Bowes、BHK、Ratec、RK13、Vero)对Leyig细胞产生一种具有类似生物学效应的因子。尽管如此，FSH和DBcAMP刺激Sertoli细胞培养产生这种因子的观察结果表明，在睾丸中，它可能在间质细胞功能的旁分泌控制中发挥作用。
We investigated whether Sertoli cell spent media (SCM) contain a factor (or factors) which influences steroidogenesis in Leydig cells. Freshly prepared prepubertal interstitial cells or Percoll-purified Leydig cells and similar cells cultured in the presence or absence of LH were incubated for 24 h in the presence of a 5α-reductase inhibitor and in the presence or absence of SCM. The accumulation of19-steroids (Testosterone and androstenedione), C21-steroids (progesterone, 17α-hydroxyprogesterone and 20α-hydroxypregn-4-en-3-one) and cyclic AMP was measured by radioimmunoassay. It could be demonstrated that SCM contains a factor that stimulates an early step in the steroidogenic pathway but at the same time hampers the conversion of C21-precursors into androgens. In freshly prepared Leydig cells the final effect is a stimulation of the androgen output. In Leydig cells cultured in the absence of LH, mainly C21-steroid output in increased. These biological effects resemble those observed with LHRH and its agonists. The activity of the Sertoli cell factor is not affected by an LHRH antagonist, however, and maximally effective concentrations of the factor and LHRH have additive effects, suggesting that they act by distinct receptor systems. Preliminary characterization shows that the factor in SCM is a thermolabile protein with a MW > 10 000. The production of the factor decreases during prolonged culture in serum-free medium. Addition of feta; calf serum causes a marked and dose-dependent increase in the production or activity of the factor. Several permanent cell lines (B16, Bowes, BHK, Ratec, RK13, Vero) produce a factor with comparable biological effects on Leydig cells. Nonetheless, the observation that the production of this factor by Sertoli cell cultures is stimulated by FSH and dbcAMP suggests that, in the testis, it may play a role in the paracrine control of Leydig cell function.
GnRH 诱导睾丸促性腺激素释放激素 (GnRH) 受体：垂体激素的作用及其与睾酮产生抑制的关系。
影响因子：
4.8
作者：
Bourne,GA;Dockrill,MR;Regiani,S;Marshall,JC;Payne,AH
通讯作者：
Payne,AH