Febrile temperatures can synchronize the growth of Plasmodium falciparum in vitro.
Febrile temperatures can synchronize the growth of Plasmodium falciparum in vitro.
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高温温度可以在体外同步恶性疟原虫的生长。
DOI：
10.1084/jem.169.1.357
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发表时间：
1989-01-01
期刊：
影响因子：
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通讯作者：
Kwiatkowski D
中科院分区：
文献类型：
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作者：
Kwiatkowski D
为了探讨疟疾中宿主发热反应可能影响疟原虫发育的可能性，我们研究了温度对体外红细胞培养的恶性疟原虫的影响。与37 ℃相比，40 ℃下生长明显受到抑制，这是由于48小时红细胞周期的后半部分被破坏。然而，年轻的红细胞内寄生虫在自然感染期间高度暴露于发热，在40摄氏度下似乎正常发育。由于红细胞周期内的温度敏感性不同，异步培养可以通过瞬时升高温度来同步。当培养物暴露于模拟间日疟48小时发热周期的温度周期性升高时，观察到明显的同步。这些发现表明，疟疾热可能会促进体内寄生虫同步。
To investigate the possibility that the host fever response in malaria may affect parasite development, we studied the effect of temperature on Plasmodium falciparum in erythrocytic culture in vitro. Growth was markedly suppressed at 40 degrees C compared with 37 degrees C, due to disruption of the second half of the 48-h erythrocytic cycle. However, young intraerythrocytic parasites, which are highly exposed to fever during natural infection, appeared to develop normally at 40 degrees C. Because of the differential temperature sensitivity within the erythrocytic cycle, asynchronous cultures could be synchronized by transient elevations of temperature. Pronounced synchronization was observed when cultures were exposed to periodic elevations of temperature that simulated the 48-h fever cycle of tertian malaria. These findings indicate that malaria fever might act to promote parasite synchronization in vivo.