Comparative analysis of the liver and plasma proteomes as a novel and powerful strategy for hepatocellular carcinoma biomarker discovery.
Comparative analysis of the liver and plasma proteomes as a novel and powerful strategy for hepatocellular carcinoma biomarker discovery.
复制标题
肝脏和血浆蛋白质组的比较分析是肝细胞癌生物标志物发现的一种新颖而有力的策略。
DOI：
10.1016/j.canlet.2009.01.025
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发表时间：
2009-12-01
期刊：
影响因子：
9.7
通讯作者：
Beretta L
中科院分区：
文献类型：
--
作者：
Beretta L
The extraordinary developments made in the past decade in proteomic technologies, in particular in mass spectrometry, have enabled investigators to consider designing studies to search for diagnostic and therapeutic biomarkers by scanning complex proteome samples. We developed a method based on extensive fractionation of intact proteins, to comprehensively and quantitatively profile the liver and plasma proteomes in health and disease. We have applied this method to samples collected from patients with early hepatocellular carcinoma (HCC) and from patients with liver cirrhosis as well as to samples collected from three mouse models of HCC. This method allowed for the identification of proteins that differ in expression levels in liver tissue or in plasma with disease progression from liver fibrosis, cirrhosis or steatohepatitis to HCC. The comparative analysis of the liver and plasma proteomes generated from human and mouse specimen, constitutes a novel and powerful strategy for HCC biomarker discovery.
