Effect of maternal antibodies on influenza virus-specific immune response elicited by inactivated virus and naked DNA.
Effect of maternal antibodies on influenza virus-specific immune response elicited by inactivated virus and naked DNA.
复制标题
母体抗体对灭活病毒和裸DNA引起的流感病毒特异性免疫反应的影响。
DOI：
10.1046/j.1365-3083.2001.00896.x
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发表时间：
2001
影响因子：
3.7
通讯作者：
Bona,C
中科院分区：
文献类型：
--
作者：
Radu,DL;Antohi,S;Bot,A;Miller,A;Mirarchi,A;Bona,C
虽然疫苗对成人有效，但对新生儿和婴儿效果较差。新生儿对疫苗无反应可能是由于淋巴细胞不成熟和/或母源抗体的抑制。新生儿对疫苗无反应可以通过基因免疫来克服。在本研究中，我们研究了母源抗体对用含有血凝素基因或紫外线灭活病毒的质粒免疫的母鼠所生后代的抗流感病毒保护反应的影响。在先用病毒或质粒免疫并与 C57BL/6 雄性杂交的 BALB/cdams 所生的质粒免疫 F1 小鼠中，以及在用质粒免疫然后用紫外线灭活的 WSN 病毒免疫的 BALB/cdams 所生的后代中研究了母源抗体的作用。我们发现，DNA免疫的母鼠所生的后代的抗HA抗体反应的抑制期比病毒免疫的母鼠所生的后代要短。此外，在母源抗体滴度下降后，经病毒免疫或注射抗病毒单克隆抗体（MoAb）的母鼠所生的后代对B细胞有持续的抑制作用。对用灭活流感病毒或质粒免疫的母鼠所生后代的血凝素特异性克隆型反应模式的分析表明，与用病毒免疫的母鼠所生的后代相比，对免疫病毒株特异性产生抗体的克隆型在用DNA免疫的母鼠所生的后代中占主导地位。母体抗体不影响细胞介导的免疫。这些发现可用于为新生儿和婴儿设计有效的疫苗接种计划。
While vaccines are effective in adults, they are less successful in newborns and infants. Neonatal unresponsiveness to vaccines could be owing to immaturity of lymphocytes and/or to inhibition by maternal antibodies. Unresponsiveness of newborn to vaccines can be overcame by genetic immunization. In the present study we investigated the effect of maternal antibodies on the anti‐influenza virus protective response in progeny born to dams immunized with plasmid containing the hemagglutinin gene or UV‐inactivated virus. The effect of maternal antibodies was studied in plasmid immunized F1 mice born to BALB/cdams, previously immunized with virus or plasmid and crossed with C57BL/6 males, as well as in offspring born to BALB/cdams immunized with plasmid and then immunized with UV‐inactivated WSN virus. We have found that the inhibition period of the anti‐HA antibody response in offspring born to dams immunized with DNA is shorter than that of offspring born to dams immunized with virus. Furthermore, there is a persistent inhibitory effect on B cells from offspring born to dams immunized with virus or injected with antiviral monoclonal antibodies (MoAb), after the decline of maternal antibody titers. The analysis of the haemagglutinin‐specific clonotype reactivity pattern of offspring born to dams immunized with inactivated influenza virus or with a plasmid showed that clonotypes producing antibodies specific for the immunizing virus strain were predominant in offspring born to dams immunized with DNA compared to those born to dams immunized with virus. Maternal antibodies do not affect cell‐mediated immunity. These findings might be used to design efficient vaccination schedules for newborns and infants.