SELECTIVE SOLID MEDIA FOR ISOLATING AND ENUMERATING ACIDOPHILIC BACTERIA
SELECTIVE SOLID MEDIA FOR ISOLATING AND ENUMERATING ACIDOPHILIC BACTERIA
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DOI：
10.1016/0167-7012(95)00015-d
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发表时间：
1995-08-01
影响因子：
2.2
通讯作者：
JOHNSON, DB
中科院分区：
文献类型：
--
作者：
JOHNSON, DB
已发表的为T.氧化亚铁和其他嗜酸微生物。已经发现许多指定的介质对于从环境样品（酸性矿井排水等）中计数和分离嗜酸菌都不令人满意。以及实验室中生长的化能无机营养生物的某些物种和菌株。然而，“覆盖”技术，其中嗜酸性异养细菌被纳入下层的两层凝胶，已被成功地用于培养所有已知的铁氧化嗜温和中度嗜热细菌。原始培养基的变体（FeSO），其中包括连四硫酸盐以及亚铁和胰蛋白胨大豆肉汤，进行了描述。该培养基支持氧化硫硫杆菌以及铁氧化嗜温菌的生长（T.氧化亚铁细菌和氧化亚铁纤梭菌（Leptoacillum ferrooxidans）和许多异养分离株，并且有助于基于菌落特征的分离株的区分和鉴定。培养基FeSO也已用于培养一系列中度嗜热嗜酸微生物。描述了第二种新型固体培养基（WAYE），用于从环境中分离和计数贫营养、异养嗜酸菌。该非覆盖培养基含有酵母提取物（0.02% w/v），并通过水洗或酸洗琼脂糖凝胶化。使用这些新的媒体电镀矿沃茨和实验室培养的数据记录结果，并总结了哪些媒体是最适合针对特定群体的嗜酸微生物。
Published solid media formulations designed for T. ferrooxidans and other acidophilic microorganisms are reviewed. Many designated media have been found to be unsatisfactory both for enumerating and isolating acidophiles from environmental samples (acid mine drainage etc.) and for some species and strains of chemolithotrophs grown in the laboratory. However, the 'overlay' technique, in which an acidophilic heterotrophic bacterium is incorporated into the underlayer of a two-layer gel, has been used successfully to culture all known iron-oxidising mesophilic and moderately thermophilic bacteria. A variant (FeSo) of the original medium, which includes tetrathionate as well as ferrous iron and tryptone soya broth, is described. This medium supports the growth of Thiobacillus thiooxidans as well as iron-oxidising mesophiles (T. ferrooxidans and Leptospirillum ferrooxidans) and many heterotrophic isolates, and facilitates differentiation and identification of isolates based on colony characteristics. Medium FeSo has also been used to culture a range of moderately thermophilic acidophilic microorganisms. A second novel solid medium is described (WAYE) for isolating and enumerating oligotrophic, heterotrophic acidophiles from the environment. This non-overlay medium contains yeast extract (at 0.02% w/v) and is gelled by water- or acid-washed agarose. Data recording results from using these novel media for plating mine waters and laboratory cultures are presented, and a summary is made of which media are most appropriate for targeting specific groups of acidophilic microorganisms.