Structure of secondary cleavage sites of E. coli RNAaseIII in A3t RNA from bacteriophage T7.
Structure of secondary cleavage sites of E. coli RNAaseIII in A3t RNA from bacteriophage T7.
复制标题
噬菌体 T7 的 A3t RNA 中大肠杆菌 RNAaseIII 二级切割位点的结构。
作者：
G. Gross;J. Dunn
描述了来自噬菌体 T7 的起始 RNA A3t（141 个核苷酸）内大肠杆菌 RNAaseIII 的五个“二级”切割位点。切割显然发生在一段短的双链 RNA 内部或底部。三个二级位点在镁 2+ 离子存在下被有效裂解，另外两个位点在低一价盐浓度（小于 0.05 M）的锰 2+ 离子存在下被裂解。
Five 'secondary' cleavage sites of E. coli RNAaseIII within the initiator RNA A3t (141 nucleotides) from bacteriophage T7 are described. Cleavage takes place apparEntly within or at the bottom of a short double-stranded stretch of RNA. Three secondary sites are efficiently cleaved in the presence of magnesium 2+ ions, two additional sites are cleaved in the presence of manganese 2+ ions at low monovalent salt concentrations (less than 0.05 M).