Characterizing the Cytocompatibility of Various Cross-Linking Chemistries for the Production of Biostable Large-Pore Protein Crystal Materials
Characterizing the Cytocompatibility of Various Cross-Linking Chemistries for the Production of Biostable Large-Pore Protein Crystal Materials
复制标题
DOI：
10.1021/acsbiomaterials.8b00023
复制
发表时间：
2018-03-01
影响因子：
5.8
通讯作者：
Snow, Christopher D.
中科院分区：
文献类型：
--
作者：
Hartje, Luke F.;Bui, Hieu T.;Snow, Christopher D.
随着对治疗性大分子、靶向药物递送和体内生物传感的兴趣迅速增长，需要能够储存大分子和计量释放的新型纳米结构生物材料，其表现出稳健的稳定性和细胞相容性。这种材料的一种新的可能性是工程化的大孔蛋白质晶体（LPC）。在此，使用三种交联剂：戊二醛、草醛和1-乙基-3-（3-（二甲基氨基）丙基）碳二亚胺生成各种化学稳定的LPC衍生生物材料。随后评价LPC的生物稳定性和体外哺乳动物细胞相容性，并将其与类似交联的tetraphylenegg白色溶菌酶晶体进行比较。本研究证明了各种交联化学物质在物理上稳定LPC材料分子结构的能力-增加了其对挑战性条件的耐受性，同时表现出最小的细胞毒性。这种方法产生的LPC衍生的生物材料具有广阔的应用前景，在生物技术和纳米医学的各种应用。
With rapidly growing interest in therapeutic macromolecules, targeted drug delivery, and in vivo biosensing comes the need for new nanostructured biomaterials capable of macromolecule storage and metered release that exhibit robust stability and cytocompatibility. One novel possibility for such a material are engineered large-pore protein crystals (LPCs). Here, various chemically stabilized LPC derived biomaterials were generated using three cross-linking agents: glutaraldehyde, oxaldehyde, and 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide. LPC biostability and in vitro mammalian cytocompatibility was subsequently evaluated and compared to similarly cross-linked tetragonal hen egg white lysozyme crystals. This study demonstrates the ability of various cross-linking chemistries to physically stabilize the molecular structure of LPC materials-increasing their tolerance to challenging conditions while exhibiting minimal cytotoxicity. This approach produces LPC-derived biomaterials with promising utility for diverse applications in biotechnology and nanomedicine.