Gaining insight into cellular cardiac physiology using single particle tracking
Gaining insight into cellular cardiac physiology using single particle tracking
复制标题
使用单粒子跟踪深入了解细胞心脏生理学
DOI：
10.1016/j.yjmcc.2020.08.011
复制
发表时间：
2020
影响因子：
5
通讯作者：
Liu Jie
中科院分区：
文献类型：
--
作者：
Ying Li;Jing Yi;Wenjuan Liu;Yun Liu;Liu Jie
单粒子跟踪（SPT）是一种直接监测活细胞中单分子行为的可靠技术。通过这种方法，我们可以通过统计表征单个分子行为来揭示粒子动力学的潜在生物学意义。SPT在单分子水平上提供了有价值的信息，这些信息可能被传统系综测量固有的简单平均所掩盖。在这里，我们简要介绍了SPT，包括常用的光学实现，荧光标记策略和数据分析方法。然后，我们关注如何利用SPT来破译心肌功能。在此背景下，SPT为信号受体和离子通道的横向扩散、心脏纳米结构域的动态组织、心脏离子通道的亚基组成和化学计量、肌凝蛋白沿着肌动蛋白丝的运动、参与心脏重构的转录因子的动力学特征以及纳米管的细胞间通讯提供了新的见解。最后，我们推测了应用SPT来解决关于细胞心脏生理学的未来问题的前景和挑战。
Single particle tracking (SPT) is a robust technique to monitor single-molecule behaviors in living cells directly. By this approach, we can uncover the potential biological significance of particle dynamics by statistically characterizing individual molecular behaviors. SPT provides valuable information at the single-molecule level, that could be obscured by simple averaging that is inherent to conventional ensemble measurements. Here, we give a brief introduction to SPT including the commonly used optical implementations, fluorescence labeling strategies, and data analysis methods. We then focus on how SPT has been harnessed to decipher myocardial function. In this context, SPT has provided novel insight into the lateral diffusion of signal receptors and ion channels, the dynamic organization of cardiac nanodomains, subunit composition and stoichiometry of cardiac ion channels, myosin movement along actin filaments, the kinetic features of transcription factors involved in cardiac remodeling, and the intercellular communication by nanotubes. Finally, we speculate on the prospects and challenges of applying SPT to future questions regarding cellular cardiac physiology using SPT.