EFFECTS OF THE 2 VARIETIES OF CRYPTOCOCCUS-NEOFORMANS CELLS AND CULTURE FILTRATE ANTIGENS ON NEUTROPHIL LOCOMOTION
EFFECTS OF THE 2 VARIETIES OF CRYPTOCOCCUS-NEOFORMANS CELLS AND CULTURE FILTRATE ANTIGENS ON NEUTROPHIL LOCOMOTION
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DOI：
10.1128/iai.63.7.2632-2644.1995
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发表时间：
1995-07-01
影响因子：
3.1
通讯作者：
MURPHY, JW
中科院分区：
文献类型：
--
作者：
DONG, ZM;MURPHY, JW
新型隐球菌gattii（血清型B和C）分离株对免疫活性宿主具有相对偏好，而C.新形变种新生儿（血清型A和D）分离株对免疫功能低下的宿主有相对的偏好，这表明正常宿主对前者的抗性可能比对后者的抗性相对无效。为了评估正常的细胞宿主防御不足以抵抗C。新形变种gattii，比较了两个品种的C.在48孔改良Boyden小室中，观察了新生隐球菌细胞及其培养滤液抗原（CneF）对中性粒细胞运动的影响。新形变种新生儿（血清型A和D）分离物刺激人PMN的趋化性和趋化作用，并激活合并的人血清中的补体成分，使其成为人PMN的化学引诱物。而C.新形变种gattii（血清型B和C）分离株不刺激人PMN的趋化性或趋化运动，而是抑制PMN对合并的人血清和甲酰甲硫氨酰亮氨酰苯丙氨酸的趋化运动和趋化反应。新生隐球菌格特变种CneF对PMN无细胞毒性作用。新形变种neoformans引起PMN迁移到植入幼稚和免疫小鼠的明胶海绵中，而来自C.新形变种gattii抑制PMN迁移到海绵。我们的研究结果，结合其他人的发现，表明减少中性粒细胞浸润的小鼠肺感染C。新形变种gattii感染小鼠肺内PMN浸润的变化。新形变种neoformans，表明正常的宿主抵抗机制相对不足，以防止感染与C。新形变种gattii部分是由于抑制了PMN迁移到生物体的部位。
Cryptococcus neoformans var. gattii (serotype B and C) isolates have a relative predilection for immunocompetent hosts, and C. neoformans var. neoformans (serotype A and D) isolates have a relative predilection for immunocompromised hosts, suggesting that normal host resistance to the former may be relatively inefficient compared with that to the latter variety. In order to assess the possibility that normal cellular host defense is inadequate in protecting against C. neoformans var. gattii, we compared the two varieties of C. neoformans cells and their culture filtrate antigens (CneF) with respect to effects on neutrophil (polymorphonuclear leukocyte [PMN]) locomotion, In a 48-well modified Boyden chamber, the cells and CneF of C. neoformans var. neoformans (serotype A and D) isolates stimulated chemotaxis and chemokinesis of human PMN and activated a complement component(s) in pooled human serum to become a chemoattractant(s) for human PMN. In contrast, the cells and CneF of C. neoformans var. gattii (serotype B and C) isolates did not stimulate chemotaxis or chemokinesis in human PMN but rather inhibited chemokinesis and chemotactic responses of PMN to pooled human serum and formylmethionyl leucyl phenylalanine. Neither of the CneF from the C neoformans var, gattii isolates was cytotoxic to PMN, Furthermore, with the mouse model, we found that CneF from C. neoformans var. neoformans caused migration of PMN into gelatin sponges implanted in naive and immunized mice, whereas CneF from C. neoformans var. gattii inhibited PMN migration into sponges. Our results, combined with findings of others showing reduced PMN infiltration in lungs of mice infected with C. neoformans var. gattii compared with PMN infiltration in lungs of mice infected with C. neoformans var. neoformans, indicate that the relative inadequacy of normal host resistance mechanisms to prevent infection with C. neoformans var. gattii results, in part, from inhibition of PMN migration to the site of the organism.