Molecular characterization of Llchit1, a midgut chitinase cDNA from the leishmaniasis vector Lutzomyia longipalpis
Molecular characterization of Llchit1, a midgut chitinase cDNA from the leishmaniasis vector Lutzomyia longipalpis
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DOI：
10.1016/s0965-1748(02)00209-6
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发表时间：
2003-03-01
影响因子：
3.8
通讯作者：
Traub-Csekö, YM
中科院分区：
文献类型：
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作者：
Ramalho-Ortigao, JM;Traub-Csekö, YM
在白蛉媒介的中肠内发育期间，经历分化和增殖后的利什曼原虫寄生虫必须逃离围食基质（PM）。虽然利什曼原虫几丁质酶被认为通过诱导几丁质纤维的降解参与促进寄生虫从PM逃逸，但可以想象，白蛉衍生的几丁质酶也可以在这样的事件中起作用。在这里，我们描述了一个完整的cDNA的分子克隆和部分特征，从一个假定的肠道特异性，血液诱导的几丁质酶从白蛉载体Lutzomyia longipalpis。Llchit 1具有1425 bp的ORF，其编码预测的51.6 kDa的成熟蛋白，显示出与来自几种不同生物的几丁质酶的高度相似性。信使RNA表达研究表明，Llchit 1只在血液喂养的中肠检测到，它似乎在血餐（PBM）后约72小时达到高峰。迄今为止，只有一个中肠特异性几丁质酶从昆虫疾病的载体，AgChi-1从冈比亚按蚊，已被确定。与其蚊子对应物一样，Llchit 1]可以成为开发传播阻断疫苗的靶标。(C)2003爱思唯尔科技有限公司版权所有。
During development within the midgut of the sand fly vector, Leishmania parasites after undergoing differentiation and multiplication must escape the peritrophic matrix (PM). Although Leishmania chitinase is believed to take part in promoting the escape of the parasite from the PM by inducing degradation of chitin fibers, it is conceivable that a sand fly-derived chitinase can also have a role in such an event. Here we describe the molecular cloning and partial characterization of a complete cDNA from a putative gut-specific, blood-induced chitinase from the sand fly vector Lutzomyia longipalpis. Llchit1 has an ORF of 1425 bp that encodes a predicted 51.6 kDa mature protein showing high similarity with chitinases from several different organisms. Messenger RNA expression studies indicate that Llchit1 is detected only in the blood fed midgut and it seems to reach a peak at approximately 72 h post blood meal (PBM). To date, only one midgut-specific chitinase from an insect disease vector, AgChi-1 from Anopheles gambiae, has been characterized. As with its mosquito counterpart, Llchit1] can be a target for development of a transmission blocking vaccine. (C) 2003 Elsevier Science Ltd. All rights reserved.