Pattern Formation by Staphylococcus epidermidis via Droplet Evaporation on Micropillars Arrays at a Surface
Pattern Formation by Staphylococcus epidermidis via Droplet Evaporation on Micropillars Arrays at a Surface
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DOI：
10.1021/acs.langmuir.6b01658
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发表时间：
2016-07-19
期刊：
影响因子：
3.9
通讯作者：
Rayal, R.
中科院分区：
文献类型：
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作者：
Susarrey-Arce, A.;Marin, A.;Rayal, R.
我们评估了环氧树脂表面结构化对含有表皮葡萄球菌（S.epidermidis）的水滴蒸发的影响。在蒸发过程中，含S.表皮细胞产生复杂的润湿模式，例如拉链润湿（1-3）和咖啡染色效应。取决于微结构的高度，润湿前沿以圆形或阶梯式方式传播，导致形成八边形或方形沉积图案。（4，5）我们观察到干燥液滴的形状对沉积在微柱之间的表皮的局部空间分布有相当大的影响。这些变化归因于在含S的螺旋形液滴中拉链润湿（1）和咖啡染色（6）效应之间的未探索的相互作用。表皮S蒸发过程中的诱导毛细流动。表皮用聚苯乙烯颗粒模拟。S.表皮显示出高的增殖细胞活力，但在微结构化表面上的生物量沉积低。我们的研究结果为微结构表面的开发提供了设计标准，在微结构表面上可以控制细菌繁殖，限制了杀菌剂的使用。
We evaluate the effect of epoxy surface structuring on the evaporation of water droplets containing Staphylococcus epidermidis (S.epidermidis). During evaporation, droplets with S. epidermidis cells yield to complex wetting patterns such as the zipping-wetting(1-3) and the coffee-stain effects. Depending on the height of the microstructure, the wetting fronts propagate circularly or in a stepwise manner, leading to the formation of octagonal or square-shaped deposition patterns.(4,5) We observed that the shape of the dried droplets has considerable influence on the local spatial distribution of epidermidis deposited between micropillars. These changes are attributed to an unexplored interplay between the zipping-wetting(1) and the coffee-stain(6) effects in polygonally shaped droplets containing S. epidermidis. Induced capillary flows during evaporation of S. epidermidis are modeled with polystyrene particles.. Bacterial viability measurements for S. epidermidis show high viability of planktonic cells, but low biomass deposition on the microstructured surfaces. Our findings provide insights into design criteria for the development of microstructured surfaces on which bacterial propagation could be controlled, limiting the use of biocides.