Kinetic effect of oestrogen on secretion of prostaglandins E2 and F2 in bovine oviduct epithelial cells
Kinetic effect of oestrogen on secretion of prostaglandins E2 and F2 in bovine oviduct epithelial cells
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雌激素对前列腺素E2和F2分泌的动力学影响？
DOI：
10.1071/rd15246
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发表时间：
2017-01-01
影响因子：
1.9
通讯作者：
Cao, Jinshan
中科院分区：
文献类型：
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作者：
Dong, Zhiheng;Zhang, Nan;Cao, Jinshan
本研究旨在探讨雌激素对牛输卵管上皮细胞分泌前列腺素E2 （PGE(2)）和前列腺素F2 （PGF(2)）的影响。从新鲜牛输卵管的管腔中获得牛输卵管上皮细胞。采用实时定量聚合酶链反应和细胞内western法检测PGE(2)和PGF(2)合成酶活性，采用酶联免疫吸附法检测细胞外液中两种前列腺素的浓度。我们观察到雌二醇引起环氧化酶-2 （COX-2）的短期增加，从而刺激PGE(2)和PGF(2)的分泌，随后COX-2的减少和环氧化酶-1 （COX-1）的增加产生高PGE(2):PGF(2)比率。这些发现反映了在雌激素影响下PGE(2)和PGF(2)水平的动态变化，这可能是受精所必需的。
This study aimed to investigate the effect of oestrogen on prostaglandin E2 (PGE(2)) and prostaglandin F2 (PGF(2)) secretion in bovine oviduct epithelial cells. Bovine oviduct epithelial cells were obtained from the lumen of fresh bovine oviducts. Quantitative real-time polymerase chain reaction and in-cell western assays were used to measure PGE(2) and PGF(2) synthase activity and enzyme-linked immunosorbent assays were used to detect the concentrations of the two prostaglandins in extracellular fluid. We observed that oestradiol caused a short-term increase in cyclo-oxygenase-2 (COX-2), which stimulated PGE(2) and PGF(2) secretion, and that a subsequent decrease in COX-2 and an increase in cyclo-oxygenase-1 (COX-1) produced a high PGE(2):PGF(2) ratio. These findings reflect the dynamic change in PGE(2) and PGF(2) levels under the influence of oestrogen, which may be essential for fertilisation.