Central Administration of Neuropeptide FF and Related Peptides Attenuate Systemic Morphine Analgesia in Mice
Central Administration of Neuropeptide FF and Related Peptides Attenuate Systemic Morphine Analgesia in Mice
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神经肽 FF 和相关肽的中央给药可减弱小鼠全身吗啡镇痛作用
DOI：
10.2174/092986611794654012
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发表时间：
2011-04-01
影响因子：
1.6
通讯作者：
Wang, Rui
中科院分区：
文献类型：
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作者：
Fang, Quan;Jiang, Tian-nan;Wang, Rui
神经肽Ff(NPFf)属于阿片调节肽家族。据报道，NPFF通过与阿片系统的相互作用，在控制疼痛和镇痛方面发挥重要作用。然而，很少有研究考察脊髓上NPFF系统对阿片类药物外周给药所致镇痛的影响。在本研究中，脑室内(i.c.v.)注射NPFF(1，3和10nmol)可剂量依赖性地抑制吗啡(0.12 mg，i.p.)。小鼠甩尾实验中的镇痛作用。同样，I.C.V.分别给予NPFF(2)和NPFF(1)受体的高选择性激动剂DNPA和NPVF，可降低腹腔注射的镇痛作用。小鼠体内的吗啡。此外，NPFF受体选择性拮抗剂RF9(10nmol，i.c.v.)可阻断NPFF及相关多肽的上述抗阿片活性。这些结果表明，中枢NPFF1和NPFF2受体的激活对全身吗啡的抗伤害效应具有相似的抗阿片作用。
Neuropeptide FF (NPFF) belongs to an opioid-modulating peptide family. NPFF has been reported to play important roles in the control of pain and analgesia through interactions with the opioid system. However, very few studies examined the effect of supraspinal NPFF system on analgesia induced by opiates administered at the peripheral level. In the present study, intracerebroventricular (i.c.v.) injection of NPFF (1, 3 and 10 nmol) dose-dependently inhibited systemic morphine (0.12 mg, i.p.) analgesia in the mouse tail flick test. Similarly, i.c.v. administration of dNPA and NPVF, two agonists highly selective for NPFF(2) and NPFF(1) receptors, respectively, decreased analgesia induced by i.p. morphine in mice. Furthermore, these anti-opioid activities of NPFF and related peptides were blocked by pretreatment with the NPFF receptors selective antagonist RF9 (10 nmol, i.c.v.). These results demonstrate that activation of central NPFF1 and NPFF2 receptors has the similar anti-opioid actions on the antinociceptive effect of systemic morphine.