Nutritional intervention in early life to manipulate rumen microbial colonization and methane output by kid goats postweaning
Nutritional intervention in early life to manipulate rumen microbial colonization and methane output by kid goats postweaning
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DOI：
10.2527/jas.2012-6142
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发表时间：
2013-10-01
影响因子：
3.3
通讯作者：
Yanez-Ruiz, D. R.
中科院分区：
文献类型：
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作者：
Abecia, L.;Martin-Garcia, A. I.;Yanez-Ruiz, D. R.
人们对通过饮食方式减少反刍动物甲烷 (CH4) 排放的兴趣日益浓厚，但受到成年动物瘤胃微生物群落复杂性的限制。这项工作的目的是研究对山羊幼崽生命早期的干预是否会对甲烷排放和瘤胃微生物生态系统产生影响，以及这种影响在断奶后是否持续存在。将 16 只产 2 胎的母山羊随机分为 2 个实验组：其中 8 只在产后 2 个月后接受溴氯甲烷 (BCM) 处理 (D+)，另外 8 只不进行处理 (D-)。在两组母鹿中，每只母鹿的 1 只幼崽接受 BCM (k+) 治疗 3 个月，另一只不接受治疗 (k-)，产生 4 个实验组：D+k+、D+k-、D-k+ 和 D-k-。记录甲烷排放量，并在 2 个月大（断奶，W）以及 1（W+1）和 4（W+4）个月后收集瘤胃样本。第 W+1 个月时，k+ 儿童的 CH4 排放量比未治疗的儿童（分别为 D+ 和 D-组）少 52% 和 59%。然而，在第 W+4 个月时，与其他 3 组（121.8 克/天）相比，只有 D+k+ 儿童的发射量仍然较低（33%），并且每日体重增加量更高（146 克/天）。通过变性梯度凝胶电泳 (DGGE) 对古菌群落结构的分析表明，BCM 治疗对母兔和幼崽有很强的影响，这种影响仅在 D+k+ 幼崽中持续存在。研究表明，在儿童生命早期应用 BCM 改变了瘤胃中的古菌种群，从而导致断奶前后 CH4 排放量减少。该效果受到母鹿所接受治疗的影响，并且在 D+ k+ 儿童中持续 3 个月。
The growing interest in reducing methane (CH4) emissions from ruminants by dietary means is constrained by the complexity of the microbial community in the rumen of the adult animal. The aim of this work was to study whether intervention in early life of goat kids has an impact on methane emissions and the microbial ecosystem in the rumen and whether the effects persist postweaning. Sixteen doe goats giving birth to 2 kids each were randomly split into 2 experimental groups: 8 does were treated (D+) with bromochloromethane (BCM) after giving birth and over 2 mo, and the other 8 does were not treated (D-). In both groups of does, 1 kid per doe was treated with BCM (k+) for 3 mo, and the other was untreated (k-), resulting in 4 experimental groups: D+k+, D+k-, D-k+, and D-k-. Methane emissions were recorded, and ruminal samples were collected from kids at 2 mo of age (weaning, W) and 1 (W+1) and 4 (W+4) mo later. At W+1 mo, CH4 emissions by k+kids were 52% and 59% less than untreated kids (in D+ and D-groups, respectively). However, at W+4 mo, only D+k+ kids remained lower (33%) emitters and exhibited greater daily BW gain (146 g/d) compared with the other 3 groups (121.8 g/d). The analysis of the archaeal community structure by Denaturing Gradient Gel Electrophoresis (DGGE)showed a strong effect of BCM treatment on does and kids that persisted only in D+k+ kids. The study showed that the application of BCM during early life of kids modified the archaeal population that colonized the rumen, which resulted in decreased CH4 emissions around weaning. The effect is influenced by the treatment applied to the doe and persisted 3 mo later in D+ k+ kids.