Single-amino-acid substitutions within the signal sequence of yeast prepro-alpha-factor affect membrane translocation.
Single-amino-acid substitutions within the signal sequence of yeast prepro-alpha-factor affect membrane translocation.
复制标题
酵母前α因子原信号序列内的单氨基酸取代会影响膜易位。
DOI：
10.1128/mcb.8.5.1915-1922.1988
复制
发表时间：
1988
影响因子：
5.3
通讯作者：
Young,ET
中科院分区：
文献类型：
--
作者：
Allison,DS;Young,ET
We used a genetic approach to identify point mutations in the signal sequence of a secreted eucaryotic protein, yeast α-factor. Signal sequence mutants were obtained by selecting for cells that partially mistargeted into mitochondria a fusion protein consisting of the α-factor signal sequence fused to the mature portion of an imported mitochondrial protein (Cox IV). The mutations resulted in replacement of a residue in the hydrophobic core of the signal sequence with either a hydrophilic amino acid or a proline. After reassembly into an intact α-factor gene, the substitutions were found to decrease up to 50-fold the rate of translocation of prepro-α-factor across microsomal membranes in vitro. Two of three mutants tested produced lower steady-state levels of α-factor in intact yeast cells, although the magnitude of the effect was less than that in the cell-free system.
