A PROMOTER THAT DRIVES TRANSGENE EXPRESSION IN CEREBELLAR PURKINJE AND RETINAL BIPOLAR NEURONS
A PROMOTER THAT DRIVES TRANSGENE EXPRESSION IN CEREBELLAR PURKINJE AND RETINAL BIPOLAR NEURONS
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DOI：
10.1126/science.2109351
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发表时间：
1990-04-13
期刊：
影响因子：
56.9
通讯作者：
MORGAN, JI
中科院分区：
文献类型：
--
作者：
OBERDICK, J;SMEYNE, RJ;MORGAN, JI
编码浦肯野细胞特异性L7蛋白的基因组克隆已被分离并用于驱动β-L7蛋白的表达。小鼠的半乳糖苷酶。三个独立的转基因系，用L7-β-L7转化的生殖系，半乳糖苷酶融合基因，表现出β-半乳糖苷酶在小脑浦肯野细胞和视网膜双极神经元中的表达。该分布与先前通过免疫组织化学测定的L7蛋白的分布相同。转基因鼠系可用于获得标记的浦肯野和双极神经元的群体。类似的L7启动子构建体可用于在这两类神经元中特异性表达其他外源基因。
A genomic clone encoding the Purkinje cell-specific L7 protein has been isolated and utilized to drive the expression of .beta.-galactosidase in mice. Three independent transgenic lines, germ line transformed with an L7-.beta.-galactosidase fusion gene, exhibit .beta.-galactosidase expression in both cerebellar Purkinje cells and retinal bipolar neurons. This distribution is the same as that previously determined for the L7 protein by immunohistochemistry. The transgenic murine lines can be used to obtain populations of marked Purkinje and bipolar neurons. Similar L7 promoter constructs can be used to express other foreign genes specifically in these two classes of neurons.