Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells
Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells
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DOI：
10.1021/acssynbio.8b00365
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发表时间：
2019-05-01
影响因子：
4.7
通讯作者：
Ouyan, Hongsheng
中科院分区：
文献类型：
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作者：
Dong, Haisi;Su, Ang;Ouyan, Hongsheng
猪瘟是一种高度传染性的猪传染病，造成毁灭性的经济损失。然而，几乎没有针对CSF病毒（CSFV）的有效治疗性抗体。因此，我们直接从使用CSFV E2蛋白和山羊抗猪IgG的保守线性表位分选的单个B细胞中分离了两种全猪抗CSFV中和抗体（NAb）。这些单克隆抗体，称为HK 24和HK 44，可以通过识别E2的保守线性表位内的位点与E2蛋白结合。此外，这两种单克隆抗体可以检测病毒感染具有高度的特异性，并具有强大的中和活性。HK 24和HK 44在体外保护PK-15细胞免受CSFV感染，有效的IC 50值分别为9.3和0.62 μ g/mL。我们预期这些抗体可用作CSFV的诊断和抗病毒剂，并且我们在此描述的方法将加速其他病毒的治疗性抗体的产生。
Classical swine fever (CSF) is a highly contagious swine disease that causes devastating economic losses. However, there are few efficacious therapeutic antibodies against the CSF virus (CSFV). Accordingly, we isolated two whole-porcine anti-CSFV neutralizing antibodies (NAbs) directly from single B cells sorted using the conserved linear epitope of the CSFV E2 protein and goat anti-pig IgG. These mAbs, termed HK24 and HK44, can bind to the E2 protein by recognizing sites within the conserved linear epitope of E2. In addition, these two mAbs can detect virus infection with high specificity and possess potent neutralizing activity. HK24 and HK44 protect PK-15 cells from CSFV infections in vitro with potent IC50 values of 9.3 and 0.62 mu g/mL, respectively. We anticipate that these antibodies can be used as diagnostic and antiviral agents for CSFV and that the method we describe here will accelerate the production of therapeutic antibodies for other viruses.