Distribution of insulin receptors between plasmalemma and intracellular compartments: effect of amino acids.
Distribution of insulin receptors between plasmalemma and intracellular compartments: effect of amino acids.
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胰岛素受体在质膜和细胞内区室之间的分布：氨基酸的作用。
DOI：
10.1002/jcp.1041220118
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发表时间：
1985
影响因子：
5.6
通讯作者：
Buse,MG
中科院分区：
文献类型：
--
作者：
Galbraith,RA;Buse,MG
在原代培养的大鼠成红细胞白血病（EBL）细胞中研究了胰岛素受体的调节。在含有12种必需氨基酸、谷氨酰胺和丝氨酸的培养基中孵育1-2小时后，与不含丝氨酸的细胞相比，EBL细胞蛋白质合成和胰岛素受体浓度增加。在丝氨酸的存在下，培养基异亮氨酸的缺乏迅速降低了蛋白质合成和胰岛素与完整细胞的结合。补充丝氨酸或异亮氨酸的缺乏培养基对溶解细胞制剂中测得的总胰岛素受体数量没有影响。在4 ° C和37°C的结合试验中观察到丝氨酸暴露后胰岛素结合增加。在37°C结合测定后定量细胞内配体的解离实验显示表面结合和细胞内[125 I]胰岛素积累均增加。这些数据与以前的观察结果相结合表明，该细胞必需的氨基酸是快速合成不稳定的调节蛋白所必需的，该调节蛋白促进胰岛素受体的再分布和/或再循环。
Insulin receptor regulation was studied in the rat erythroblastic leukemic (EBL) cell in primary culture. After 1–2‐hr incubations in medium containing 12 essential amino acids, glutamine, and serine, EBL cell protein synthesis and insulin receptor concentrations were increased compared to cells incubated without serine. Deficiency of medium isoleucine in the presence of serine rapidly decreased protein synthesis and insulin binding to intact cells. Supplementation of deficient media with serine or isoleucine had no effect on total insulin receptor numbers measured in solubilized cell preparations. Increased insulin binding following serine exposure was seen with binding assays at both 4 and 37°C. Dissociation experiments to quantitate intracellular ligand after 37°C binding assays showed increases in both surface binding and intracellular [125I]insulin accumulation. These data combined with previous observations suggest that amino acids essential for this cell are required for the rapid synthesis of a labile regulatory protein which facilitates the redistribution and/or recycling of insulin receptors.