Oxygen activating nonheme iron enzymes
Oxygen activating nonheme iron enzymes
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DOI：
10.1016/s1367-5931(98)80057-4
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发表时间：
1998-04-01
影响因子：
7.8
通讯作者：
Que, L
中科院分区：
文献类型：
--
作者：
Lange, SJ;Que, L
在过去的一年里，氧激活非血红素铁酶的研究取得了重大进展。原儿茶酸3，4-双加氧酶底物和底物类似物的13个晶体结构揭示了催化过程中酶活性部位的变化。结晶学数据已经证实，2-His-1-Cxylate面部三联体是许多单核非血红素铁酶的共同结构基序，包括异青霉素N合成酶、酪氨酸羟基酶和萘双加氧酶。首次获得了甲烷单加氧酶和核糖核苷酸还原酶的高价中间体Q和X的测量数据。被认为具有非血红素二铁位点的酶的数量已经扩大到包括来自Xanthbacter菌株Py2的烯烃单加氧酶和来自油假单胞菌的膜结合的烷烃羟基酶(AtkB)。最后，合成的络合物成功地模拟了单核和双核非血红素铁酶的化学行为，如外切解儿茶酚双加氧酶、脂肪加氧酶、烷烃和烯烃单加氧酶以及脂肪酸去饱和酶。
The past year has witnessed significant advances in the study of oxygen-activating nonheme iron enzymes. Thirteen crystal structures of substrate and substrate analog complexes of protocatechuate 3,4-dioxygenase have revealed intimate details about changes at the enzyme active site during catalysis. Crystallographic data have established a 2-His-1-carboxylate facial triad as a structural motif common to a number of mononuclear nonheme iron enzymes, including isopenicillin N synthase, tyrosine hydroxylase and naphthalene dioxygenase. The first metrical data has been obtained for the high valent intermediates Q and X of methane monooxygenase and ribonucleotide reductase, respectively. The number of enzymes thought to have nonheme diiron sites has been expanded to include alkene monooxygenase from Xanthobacter strain Py2 and the membrane-bound alkane hydroxylase from Pseudomonas oleovorans (AtkB). Finally, synthetic complexes have successfully mimicked chemistry performed by both mono-and dinuclear nonheme iron enzymes, such as the extradiol-cleaving catechol dioxygenases, lipoxygenase, alkane and alkene monoxygenases and fatty acid desaturases.