Phenylboronic acid-functionalized core–shell magnetic composite nanoparticles as a novel protocol for selective enrichment of fructose from a fructose–glucose aqueous solution
Phenylboronic acid-functionalized core–shell magnetic composite nanoparticles as a novel protocol for selective enrichment of fructose from a fructose–glucose aqueous solution
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DOI：
10.1039/c7nj02106b
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发表时间：
2017-11
影响因子：
3.3
通讯作者：
G. Lei;Yun Wang;Juan Han;Lei Wang;Xu Tang;Cheng Li;Liang Ni
中科院分区：
文献类型：
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作者：
G. Lei;Yun Wang;Juan Han;Lei Wang;Xu Tang;Cheng Li;Liang Ni
我们开发了一种高效、温和的制备硼酸功能化磁性纳米粒子（MNP）的方法，并首次证明了从样品溶液中选择性分离果糖。采用改进的水热法制备了裸Fe 3 O 4纳米粒子，然后通过溶胶-凝胶法合成了Fe3O4@SiO2纳米粒子。在此基础上，采用溶胶-凝胶法，以有机硅偶联剂为偶联剂，制备了巯基包覆的Fe 3 O 4纳米粒子。最后，MNP表面上的硫醇基团充当可点击位点，通过硫醇-烯（TE）点击反应与4-乙烯基苯基硼酸（VPBA）反应，以获得具有高密度的硼酸配体固定在表面上的最终MNP。已知Fe3O4@SiO2@VPBA MNP上的苯基硼酸配体可以与含顺式二醇的分子形成稳定的五元或六元环酯。在这项工作中，点击Fe 3 O 4 MNP的平均直径为195 nm，表现出高的选择性和结合能力，对果糖。在最佳吸附条件下，果糖的吸附率可达96%以上。此外，在果糖-葡萄糖水溶液中，Fe3O4@SiO2@VPBA MNP对果糖的吸附效率约为葡萄糖的6倍，表明在竞争葡萄糖的干扰下，Fe3O4@SiO2@VPBA MNP对果糖具有更高的亲和力。可重复使用性也是亲和吸附剂的重要因素，Fe3O4@SiO2@VPBA MNP的吸附容量在重复使用6次后几乎没有变化。
We developed an efficient and mild method for the preparation of boronic acid-functionalized magnetic nanoparticles (MNPs), and the selective separation of fructose from a sample solution was demonstrated for the first time. A modified hydrothermal method was chosen to prepare the naked Fe3O4 MNPs, and then the Fe3O4@SiO2 MNPs were synthesized by a sol–gel reaction. After that, the thiol-coated Fe3O4 MNPs were synthesized by a sol–gel reaction with an organosilicon coupling agent. Finally, the thiol groups on the surface of the MNPs served as clickable sites to react with 4-vinylphenylboronic acid (VPBA) via a thiol–ene (TE) click reaction to obtain the final MNPs with a high density of boronic acid ligands immobilized on the surface. It is known that phenylboronic acid ligands on Fe3O4@SiO2@VPBA MNPs can form stable five- or six-membered cyclic esters with cis-diol-containing molecules. In this work, the click-Fe3O4 MNPs with a mean diameter of 195 nm exhibited high selectivity and binding capacity towards fructose. Also, the adsorption efficiency of fructose could reach ≈96% under optimum adsorption conditions. In addition, the adsorption efficiency of fructose was about six times higher than that of glucose in a fructose–glucose aqueous solution, suggesting that the resultant Fe3O4@SiO2@VPBA MNPs had higher affinity for fructose under the interference of competing glucose. Reusability is also an important factor of affinity adsorbents, and the adsorption capacity of Fe3O4@SiO2@VPBA MNPs was virtually unchanged after six cycles of reuse.