Dopamine and glutamate control area-restricted search behavior in Caenorhabditis elegans
Dopamine and glutamate control area-restricted search behavior in Caenorhabditis elegans
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DOI：
10.1523/jneurosci.1569-03.2004
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发表时间：
2004-02-04
影响因子：
5.3
通讯作者：
Maricq, AV
中科院分区：
文献类型：
--
作者：
Hills, T;Brockie, PJ;Maricq, AV
区域限制搜索（ARS）是许多动物用来定位资源的觅食策略。该行为的特点是在最后一次遇到资源后，转向频率随时间而降低。这样可以最大化在资源丰富的区域花费的时间，并在资源稀缺时将搜索扩展到更大的区域。我们证明，多巴胺能和谷氨酸能信号传导有助于线虫秀丽隐杆线虫中控制 ARS 的神经回路。多巴胺能神经元的消融消除了 ARS 行为，多巴胺受体拮抗剂雷氯必利的应用也消除了 ARS 行为。此外，ARS 还受到谷氨酸受体亚基 GLR-1 和 GLR-2 以及 EAT-4 谷氨酸囊泡转运蛋白突变的影响。有趣的是，多巴胺预孵育可以恢复多巴胺能信号缺陷线虫的行为，但不能恢复 glr-1、glr-2 或 eat-4 突变体的行为。这表明多巴胺能和谷氨酸信号在同一途径中发挥作用来调节转动频率。 GLR-1 和 GLR-2 均在运动控制电路中表达，该电路根据感觉刺激和觅食期间向前运动的持续时间来调节运动方向。我们提出了一种在线虫 ARS 机制，其中响应食物而释放的多巴胺调节运动控制电路中的谷氨酸信号，从而导致转动频率增加。
Area-restricted search (ARS) is a foraging strategy used by many animals to locate resources. The behavior is characterized by a time-dependent reduction in turning frequency after the last resource encounter. This maximizes the time spent in areas in which resources are abundant and extends the search to a larger area when resources become scarce. We demonstrate that dopaminergic and glutamatergic signaling contribute to the neural circuit controlling ARS in the nematode Caenorhabditis elegans. Ablation of dopaminergic neurons eliminated ARS behavior, as did application of the dopamine receptor antagonist raclopride. Furthermore, ARS was affected by mutations in the glutamate receptor subunits GLR-1 and GLR-2 and the EAT-4 glutamate vesicular transporter. Interestingly, preincubation on dopamine restored the behavior in worms with defective dopaminergic signaling, but not in glr-1, glr-2, or eat-4 mutants. This suggests that dopaminergic and glutamatergic signaling function in the same pathway to regulate turn frequency. Both GLR-1 and GLR-2 are expressed in the locomotory control circuit that modulates the direction of locomotion in response to sensory stimuli and the duration of forward movement during foraging. We propose a mechanism for ARS in C. elegans in which dopamine, released in response to food, modulates glutamatergic signaling in the locomotory control circuit, thus resulting in an increased turn frequency.