Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus
Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus
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DOI：
10.1007/s10529-022-03229-y
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发表时间：
2022-02-05
影响因子：
2.7
通讯作者：
Yusoff, Khatijah
中科院分区：
文献类型：
--
作者：
Dakheel, Khulood Hamid;Abdul Rahim, Raha;Yusoff, Khatijah
目的采用凝胶酶谱法检测两种噬菌体UPMK_1和UPMK_2对耐甲氧西林金黄色葡萄球菌的降解活性。方法采用BLASTP技术对多肽催化结构域进行分析。发现了许多与噬菌体蛋白相关的肽段。结果UPMK_1和UPMK_2定制序列数据库用于肽段鉴定。噬菌体UPMK_2中的生物膜降解蛋白显示出与UPMK_1相同的对多糖细胞间粘附素依赖性和不依赖于耐甲氧西林金黄色葡萄球菌（MRSA）生物膜生产者的裂解活性，UPMK_1仅具有仅限于其宿主的裂解活性。结论两种噬菌体酶均参与了MRSA噬菌体感染过程中生物膜的降解，具有良好的抗MRSA生物膜形成的酶学特性。
Objective The degradation activity of two bacteriophages UPMK_1 and UPMK_2 against methicillin-resistant Staphylococcus aureus phages were examined using gel zymography. Methods The analysis was done using BLASTP to detect peptides catalytic domains. Many peptides that are related to several phage proteins were revealed. Results UPMK_1 and UPMK_2 custom sequence database were used for peptide identification. The biofilm-degrading proteins in the bacteriophage UPMK_2 revealed the same lytic activity towards polysaccharide intercellular adhesin-dependent and independent of Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers in comparison to UPMK_1, which had lytic activity restricted solely to its host. Conclusion Both bacteriophage enzymes were involved in MRSA biofilm degradation during phage infection and they have promising enzybiotics properties against MRSA biofilm formation.