Coordinated regulation of hepatic and adipose tissue transcriptomes by the oral administration of an amino acid mixture simulating the larval saliva of Vespa species.
Coordinated regulation of hepatic and adipose tissue transcriptomes by the oral administration of an amino acid mixture simulating the larval saliva of Vespa species.
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DOI：
10.1186/s12263-016-0534-2
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发表时间：
2016
影响因子：
3.5
通讯作者：
Abe K
中科院分区：
文献类型：
--
作者：
Shinozaki F;Abe T;Kamei A;Watanabe Y;Yasuoka A;Shimada K;Kondo K;Arai S;Kumagai K;Kondo T;Abe K
VAAM是一种氨基酸混合物，模拟了黄蜂幼虫唾液的组成。VAAM增强了小鼠的身体耐力，并被运动员用作运动前的补充饮料。然而，目前还没有关于vam对自由运动动物生理影响的资料。本研究的目的是获得有关肝和脂肪组织转录组的vam依赖性调节的信息。小鼠在任意饲养条件下口服VAAM溶液、模拟酪蛋白水解物（CAAM）的氨基酸混合物或水5 d。比较VAAM-、CAAM-和水处理组的肝脏转录组，发现VAAM特异性调节代谢途径，即糖酵解和脂肪酸氧化下调，多不饱和脂肪酸合成和糖原性氨基酸利用上调。对白色和棕色脂肪组织（分别为WAT和BAT）的类似转录组学分析表明，WAT中磷脂合成上调，BAT中细胞过程负调控。由于组织转录组的协调调节暗示了这些组织共同的上游信号的存在，我们进行了独创性途径分析。该分析表明，雌激素和胰高血糖素信号在肝脏和WAT中被激活，β -肾上腺素能信号在所有三个组织中被激活。我们发现摄入vam对自由运动小鼠的多个组织转录组有影响。糖原氨基酸的利用可能在肝脏中被激活。脂肪酸转化为磷脂，而不是三酰甘油，可能在脂肪细胞中被刺激，而在BAT中观察到的影响很小。上游因素分析显示肝脏、WAT和BAT中多种激素信号被激活。我们的数据为理解vam在代谢调节中的作用提供了一些线索。本文的在线版本（doi:10.1186/s12263-016-0534-2）包含补充材料，可供授权用户使用。
VAAM is an amino acid mixture that simulates the composition of Vespa larval saliva. VAAM enhanced physical endurance of mice and have been used by athletes as a supplementary drink before exercise. However, there is no information on the effect of VAAM on the physiology of freely moving animals. The purpose of this study was to obtain information about the VAAM-dependent regulation of liver and adipose tissue transcriptomes. Mice were orally fed a VAAM solution, an amino acid mixture mimicking casein hydrolysate (CAAM) or water under ad libitum feeding conditions for 5 days. Comparisons of the hepatic transcriptome between VAAM-, CAAM-, and water-treated groups revealed a VAAM-specific regulation of the metabolic pathway, i.e., the down-regulation of glycolysis and fatty acid oxidation and the up-regulation of polyunsaturated fatty acid synthesis and glucogenic amino acid utilization. Similar transcriptomic analyses of white and brown adipose tissues (WAT and BAT, respectively) indicated the up-regulation of phospholipid synthesis in WAT and the negative regulation of cellular processes in BAT. Because the coordinated regulation of tissue transcriptomes implied the presence of upstream signaling common to these tissues, we conducted an Ingenuity Pathways Analysis. This analysis showed that estrogenic and glucagon signals were activated in the liver and WAT and that beta-adrenergic signaling was activated in all three tissues. We found that VAAM ingestion had an effect on multiple tissue transcriptomes of freely moving mice. Utilization of glycogenic amino acids may have been activated in the liver. Fatty acid conversion into phospholipid, not to triacylglycerol, may have been stimulated in adipocytes contrasting that a little effect was observed in BAT. Analysis of upstream factors revealed that multiple hormonal signals were activated in the liver, WAT, and BAT. Our data provide some clues to understanding the role of VAAM in metabolic regulation. The online version of this article (doi:10.1186/s12263-016-0534-2) contains supplementary material, which is available to authorized users.