Effects of scorpion venom on electrolyte transport by rabbit ileum.
Effects of scorpion venom on electrolyte transport by rabbit ileum.
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蝎毒对兔回肠电解质转运的影响。
DOI：
10.1152/ajpgi.1983.244.5.g501
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Hubel,KA
中科院分区：
文献类型：
--
作者：
Hubel,KA
蝎毒，使神经去极化，被用来获得进一步的证据，壁内神经影响离子运输的兔回肠。回肠上皮，剥离固有肌层，安装在通量室修改，允许电场刺激（EFS）的组织。短路电流（Isc）对毒液的反应是最迅速的serodoxin表面上，并消除河豚毒素的反应。Isc反应受毒液批号和组织内因素的影响。毒液（10微克/毫升）和EFS分别通过减少粘膜到浆膜的运动和增加浆膜到粘膜的运动引起氯化物分泌。钠转运和残留离子通量没有变化。在毒液的存在下，EFS引起的离子转运没有进一步的变化，但组织仍然响应葡萄糖和氨茶碱。早期高峰的Isc降低约40%的阿托品，这意味着乙酰胆碱，毒液释放，刺激毒蕈碱受体。用河豚毒素阻断对毒液的Isc反应进一步证明毒液使壁内神经去极化并释放引起氯化物分泌的递质。其他发射机的身份不明。
Scorpion venom, which depolarizes nerves, was used to obtain further evidence that intramural nerves affect ion transport by the rabbit ileum. Ileal epithelium, stripped of muscularis propria, was mounted in a flux chamber modified to permit electrical field stimulation (EFS) of the tissue. Response of the short-circuit current (Isc) to venom was most rapid on the serosal surface, and the response was eliminated by tetrodotoxin. Isc response was influenced by venom batch number and by factors within the tissue. Venom (10 micrograms/ml) and EFS each caused chloride secretion by reducing mucosal-to-serosal movement and by increasing serosal-to-mucosal movement. Sodium transport and residual ion fluxes did not change. In the presence of venom, EFS caused no further changes in ion transport, but tissues still responded to glucose and to aminophylline. The early peak of Isc was reduced about 40% by atropine, implying that acetylcholine, released by venom, stimulates muscarinic receptors. The blockade of the Isc response to venom with tetrodotoxin is further evidence that venom depolarizes intramural nerves and liberates transmitters that cause chloride secretion. The identity of the other transmitters is not known.