Quantification of tomato and Arabidopsis mobile RNAs trafficking into the parasitic plant Cuscuta pentagona
Quantification of tomato and Arabidopsis mobile RNAs trafficking into the parasitic plant Cuscuta pentagona
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DOI：
10.1111/nph.12439
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发表时间：
2013-12-01
期刊：
影响因子：
9.4
通讯作者：
Westwood, James
中科院分区：
文献类型：
--
作者：
LeBlanc, Megan;Kim, Gunjune;Westwood, James
MRNA从寄主到寄主的跨物种转移已有报道，但尚未定量描述或关注特定mRNA的摄取模式和命运。实时荧光PCR和RNA-Seq方法用于鉴定和表征从番茄和拟南芥寄主到五色葫芦的可移动转录本。赤霉酸不敏感(SIGAI)和组织蛋白D蛋白酶抑制剂(SIPI)的番茄转录本在被寄生植物摄取的速度上存在显著差异，但随后分布在寄生虫的茎长上。当寄生枝条离开寄主时，寄主体内的SIPI转录本浓度在最初的8小时内出现了最大的下降。拟南芥转录本的迁移率也不同，对盐诱导的锌指蛋白(AtSZF1)转录本的特定区域的分析表明，寄主mRNAs在寄主寄主中的丰度模式明显不同。寄主mRNAs在五色锦鸡儿体内的摄取和分布似乎在mRNAs之间存在差异，甚至可能与所研究的mRNAs的区域不同。我们认为，mRNAs通过多条途径进入寄生虫，或者在宿主和寄生虫之间存在其他选择性摄取和移动的机制。
The cross-species movement of mRNA from hosts to the parasitic plant Cuscuta pentagona has been reported previously, but has not been characterized quantitatively or with attention to uptake patterns and the fate of specific mRNAs.Real-time PCR and RNA-Seq approaches were used to identify and characterize mobile transcripts from tomato and Arabidopsis hosts into C. pentagona.Tomato transcripts of Gibberellic Acid Insensitive (SIGAI) and Cathepsin D Proteinase Inhibitor (SIPI) differed significantly in the rate of uptake into the parasite, but were then distributed over the length of the parasite shoot. When parasite shoots were detached from the hosts, the SIPI transcript concentrations in the parasite showed the greatest decrease within the first 8 h. Arabidopsis transcripts also varied in mobility into the parasite, and assay of specific regions of a Salt-inducible Zinc Finger Protein (AtSZF1) transcript revealed distinct patterns of abundance in the parasite.The uptake and distribution of host mRNAs into C. pentagona appears to vary among mRNAs, and perhaps even with the region of the mRNA under investigation. We propose that mRNAs traffic into the parasite via multiple routes, or that other mechanisms for selective uptake and mobility exist between host and parasite.