Inflammatory Serum Protein Profiling of Patients with Lumbar Radicular Pain One Year after Disc Herniation
Inflammatory Serum Protein Profiling of Patients with Lumbar Radicular Pain One Year after Disc Herniation
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DOI：
10.1155/2016/3874964
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发表时间：
2016-01-01
影响因子：
2
通讯作者：
Gordh, Torsten
中科院分区：
文献类型：
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作者：
Moen, Aurora;Lind, Anne-Li;Gordh, Torsten
早期的研究表明，椎间盘突出后的腰椎神经根性疼痛可能与局部或全身炎症过程有关。在本研究中，我们调查了这些患者的血清炎症蛋白谱。所有45例患者均在2007-2009年期间从挪威Ulleval的奥斯陆大学医院招募。采用新的多重邻近延伸分析（PEA）技术分析了92种蛋白质的水平。有趣的是，目前的数据显示，椎间盘突出12个月后发生神经根性疼痛的患者在许多可测量的血清细胞因子方面可能与其他患者不同。假设错误发现率（FDR）为0.10和0.05，我们分别确定了41和13种蛋白质，这些蛋白质在椎间盘突出后一年的严重疼痛患者中显著上调。在按估计增加排名的列表的顶部，我们发现C-X-C基序趋化因子5（CXCM 5;增加217%）、表皮生长因子（EGF;增加142%）和单核细胞趋化蛋白4（MCP-4;增加70%）。此外，慢性和恢复患者之间的血清细胞因子谱有明显的总体差异。因此，目前的结果可能是重要的未来蛋白血清分析腰椎神经根性疼痛患者的预后和治疗的选择。我们的结论是，血清蛋白可能是椎间盘突出后持续疼痛的可测量的分子标志物。
Earlier studies suggest that lumbar radicular pain following disc herniation may be associated with a local or systemic inflammatory process. In the present study, we investigated the serum inflammatory protein profile of such patients. All 45 patients were recruited from Oslo University Hospital, Ulleval, Norway, during the period 2007-2009. The new multiplex proximity extension assay (PEA) technology was used to analyze the levels of 92 proteins. Interestingly, the present data showed that patients with radicular pain 12 months after disc herniation may be different from other patients with regard to many measurable serum cytokines. Given a false discovery rate (FDR) of 0.10 and 0.05, we identified 41 and 13 proteins, respectively, which were significantly upregulated in the patients with severe pain one year after disc herniation. On the top of the list ranked by estimated increase we found C-X-C motif chemokine 5 (CXCM5; 217% increase), epidermal growth factor (EGF; 142% increase), and monocyte chemotactic protein 4 (MCP-4; 70% increase). Moreover, a clear overall difference in the serum cytokine profile between the chronic and the recovered patients was demonstrated. Thus, the present results may be important for future protein serum profiling of lumbar radicular pain patients with regard to prognosis and choice of treatment. We conclude that serum proteins may be measurable molecular markers of persistent pain after disc herniation.