DIFFERENCES IN THE DETECTION AND ENUMERATION OF MUTANS STREPTOCOCCI DUE TO DIFFERENCES IN METHODS
DIFFERENCES IN THE DETECTION AND ENUMERATION OF MUTANS STREPTOCOCCI DUE TO DIFFERENCES IN METHODS
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DOI：
10.1016/0003-9969(94)00164-7
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发表时间：
1995-04-01
影响因子：
3
通讯作者：
KOHLER, B
中科院分区：
文献类型：
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作者：
DASANAYAKE, AP;CAUFIELD, PW;KOHLER, B
报告的不同方法评估变形链球菌(MS)使跨研究结果的直接比较变得困难。为了量化由于方法不同而导致的MS估计的差异，将刺激和未刺激的唾液样本以及口腔拭子样本与混合牙菌斑样本进行了比较。在刺激唾液样本中检测MS与在混合斑块样本中MS的存在具有很好的一致性。然而，未受刺激的唾液样本中的MS检测与混合菌斑或口腔拭子样本中的MS检测结果显著不一致。当以龋齿状况作为MS评估的有效性标准时，刺激唾液样本的敏感性(94%)与混合斑块样本相似，但特异性(11%)低于联合斑块样本(17%)。因此，当MS状态基于刺激唾液样本时，MS和龋齿之间的关联性的测量是有偏差的(倾向于零)。然而，有趣的是，在列举MS时，刺激的唾液样本比未刺激的唾液样本产生显著更高的MS水平(约1.5个对数(10)增加)，而变异性较低。不同的培养基对MS的检测结果也不同。当样品生长在葡萄糖-蔗糖-碲酸钾-杆菌肽琼脂上时，MS检测较差(kappa=0.31)，MS水平较低(p=0.0001)。再加上采样和处理相对容易，这些发现共同证明了使用斑块样本进行多发性硬化症定性评估和使用刺激唾液样本进行多发性硬化症定量评估的合理性，同时提供了在比较不同研究结果时调整估计的基础。
Different methods reported for assessing mutans streptococci (MS) make the direct comparisons of results across studies difficult. To quantitate the variations of MS estimates attributable to differences in method, stimulated and unstimulated saliva samples and oral swab samples were compared with pooled dental-plaque samples. Detection of MS in stimulated saliva samples was in excellent agreement with the presence of MS in pooled plaque samples. MS detection in unstimulated saliva samples, however, was significantly discordant with that in either pooled plaque or oral swab samples. When caries status was used as the criterion of validity of MS estimates, stimulated saliva samples demonstrated a sensitivity (94%) similar to that of pooled plaque samples, but exhibited a lower specificity (11%) than that of pooled plaque samples (17%). As a result, the measure of association between MS and caries was biased (towards null) when MS status was based on stimulated saliva samples. Interestingly though, in enumerating MS, stimulated saliva samples yielded significantly higher levels of MS (about 1.5 log(10) increase) with a lower variability compared to unstimulated saliva samples. The use of different culture media for detection of MS gave different results as well. MS detection was poor (kappa = 0.31) and MS levels were lower (p = 0.0001) when samples were grown on glucose-sucrose-potassium tellurite-bacitracin agar compared to mitis-salivarius-bacitracin agar. Together with the relative ease of sampling and processing, these findings collectively justify the use of plaque samples for the qualitative assessment of MS and stimulated saliva samples for the quantitative assessment of MS, while providing a basis for adjustment of estimates when comparing results across studies.