Alterations in expression and structure of the DNA repair gene XRCC1.
Alterations in expression and structure of the DNA repair gene XRCC1.
复制标题
DNA 修复基因 XRCC1 表达和结构的改变。
DOI：
10.1016/0006-291x(92)90831-5
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发表时间：
1992
影响因子：
3.1
通讯作者：
Chan,JY
中科院分区：
文献类型：
--
作者：
Yoo,H;Li,L;Sacks,PG;Thompson,LH;Becker,FF;Chan,JY
修复相关基因XRCC1先前是通过补充仓鼠突变体EM9而克隆的，该突变体具有高自发SCE率和对DNA损伤剂的超敏性。在分析 XRCC1 基因表达时，在正常细胞、SCE 改变的布卢姆综合征细胞以及 X 射线敏感性不同的鳞状癌细胞中发现了相似的稳态 mRNA 水平。先前在 XRCC1 中鉴定的 EcoRI 限制性片段长度多态性与这些细胞的修复表型不相关。用DNA损伤剂处理细胞后，XRCC1的mRNA减少至20-40%。XRCC1在大鼠中也显示出组织特异性表达。与肠道、肝脏和脾脏（1-2倍）相比，睾丸（7-8倍）、卵巢（3-4倍）和大脑（4-5倍）的mRNA水平较高。这些数据和睾丸中检测到的高水平XRCC1蛋白表明XRCC1可能在生殖细胞减数分裂和重组过程中的DNA加工中发挥重要作用。
The repair-associated geneXRCC1was previously cloned by complementing the hamster mutant EM9, which has a high rate of spontaneous SCE and hypersensitivity to DNA damaging agents. In analyzingXRCC1gene expression, similar levels of steady-state mRNA were found in normal cells, Bloom's syndrome cells with altered SCE, and in squamous carcinoma cells with differential X-ray sensitivity. AnEcoRI restriction fragment-length polymorphism previously identified inXRCC1did not correlate with the repair phenotypes of these cells. The mRNA ofXRCC1decreased to 20–40% after treatment of cells with a DNA damaging agent.XRCC1also showed tissue specific expression in rats. The mRNA levels were high in testis (7–8 fold), ovary (3–4 fold) and brain (4–5 fold), when compared with those in intestine, liver and spleen (1–2 fold). These data and the high levels ofXRCC1protein detected in testis indicate thatXRCC1may play an important role in DNA processing during meiogenesis and recombination in germ cells.