Ferredoxin 1 is downregulated by the accumulation of abscisic acid in an ABI5-dependent manner to facilitate rice stripe virus infection in Nicotiana benthamiana and rice
Ferredoxin 1 is downregulated by the accumulation of abscisic acid in an ABI5-dependent manner to facilitate rice stripe virus infection in Nicotiana benthamiana and rice
复制标题
Ferredoxin 1 通过脱落酸的积累以 ABI5 依赖性方式下调，从而促进烟草和水稻中水稻条纹病毒的感染
DOI：
10.1111/tpj.15377
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发表时间：
2021-07-10
期刊：
影响因子：
7.2
通讯作者：
Yan, Fei
中科院分区：
文献类型：
--
作者：
Cui, Weijun;Wang, Shu;Yan, Fei
铁氧还蛋白1(FD1)在植物的电子转移链中接受和分配电子。它的表达普遍受到病毒的下调，在过去的十年里，它在植物免疫中的作用已经引起了人们的关注。然而，病毒调节FD1的机制仍有待确定。在以前的报道中，我们发现在感染马铃薯X病毒(PVX)后，本氏烟草FD1(NbFD1)的表达下调，并且NbFD1调节胞间连丝的胼胝质沉积，从而起到防御PVX感染的作用。我们现在报道，NbFD1被水稻条纹病毒(RSV)感染下调，并且NbFD1的沉默也促进了RSV的感染，而在高表达NbFD1的转基因株系中病毒感染被抑制，这表明NbFD1也具有防御RSV感染的功能。接下来，鉴定了一个来自RSV的小干扰RNA，它有助于下调FD1转录本。进一步的分析表明，RSV感染植株中积累的脱落酸(ABA)也抑制了NbFD1的转录。它通过刺激ABA不敏感5(ABI5)的表达来实现这一点，ABI5与NbFD1启动子中的ABA反应元件基序结合，导致负调控。在受RSV侵染的自然寄主水稻植株中，也证实了ABA对FD1的调控。因此，这些结果表明了一种病毒调节叶绿体相关基因以抑制其防御作用的机制。
Ferredoxin 1 (FD1) accepts and distributes electrons in the electron transfer chain of plants. Its expression is universally downregulated by viruses and its roles in plant immunity have been brought into focus over the past decade. However, the mechanism by which viruses regulate FD1 remains to be defined. In a previous report, we found that the expression of Nicotiana benthamiana FD1 (NbFD1) was downregulated following infection with potato virus X (PVX) and that NbFD1 regulates callose deposition at plasmodesmata to play a role in defense against PVX infection. We now report that NbFD1 is downregulated by rice stripe virus (RSV) infection and that silencing of NbFD1 also facilitates RSV infection, while viral infection was inhibited in a transgenic line overexpressing NbFD1, indicating that NbFD1 also functions in defense against RSV infection. Next, a RSV-derived small interfering RNA was identified that contributes to the downregulation of FD1 transcripts. Further analysis showed that the abscisic acid (ABA) which accumulates in RSV-infected plants also represses NbFD1 transcription. It does this by stimulating expression of ABA insensitive 5 (ABI5), which binds the ABA response element motifs in the NbFD1 promoter, resulting in negative regulation. Regulation of FD1 by ABA was also confirmed in RSV-infected plants of the natural host rice. The results therefore suggest a mechanism by which virus regulates chloroplast-related genes to suppress their defense roles.