Release of acetylcholine by Hon-Chi to raise insulin secretion in Wistar rats
Release of acetylcholine by Hon-Chi to raise insulin secretion in Wistar rats
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DOI：
10.1016/j.neulet.2006.05.024
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发表时间：
2006-08-14
影响因子：
2.5
通讯作者：
Liu, I-Min
中科院分区：
文献类型：
--
作者：
Chen, Chang-Chih;Liu, I-Min
红曲是用红曲霉和红曲霉发酵而成的红曲米。本研究旨在筛选红芪对血糖的影响，并探讨其可能的机制。空腹Wistar大鼠口服90 min后，红芪呈剂量依赖性降低血糖。在血浆葡萄糖降低的同时，接受相同治疗的大鼠血浆胰岛素或c肽水平也有所升高。此外，使用胆碱摄取抑制剂hemicholl -3或囊泡乙酰胆碱运输抑制剂vesamicol破坏突触可用乙酰胆碱（ACh），可以消除honchi的这些作用。当毒豆碱浓度足以抑制乙酰胆碱酯酶时，其作用增强。因此可以考虑Hon-Chi介导神经末梢乙酰胆碱释放以增强胰岛素分泌。4-二苯基乙酰氧基- n-甲基哌啶甲碘醚（4-DAMP）也能抑制红芪的降血糖作用以及血浆中胰岛素和c肽水平的升高，但神经节烟碱拮抗剂、戊olinium和六甲氧铵不受影响，提示毒毒碱M-3受体的介导作用。结果表明，Hon-Chi能够提高神经末梢乙酰胆碱的释放，进而刺激胰腺细胞中的毒蕈碱M-3受体，增加胰岛素的释放，从而达到降低血糖的作用。因此，红芪可作为增加胰岛素分泌的保健食品，预防2型糖尿病。2006爱思唯尔爱尔兰有限公司版权所有。
The mandarin Hon-Chi is the red yeast rice fermented with Monascus pious and Monascus purpureus. The present study is designed to screen the effect of Hon-Chi on plasma glucose and investigate the possible mechanisms. After oral administration into fasting Wistar rats for 90 min, Hon-Chi decreased the plasma glucose in a dose-dependent manner. In parallel to the reduction of plasma glucose, an increase of plasma level of insulin or C-peptide was also observed in rats receiving same treatment. Moreover, disruption of synaptic available acetylcholine (ACh) using an inhibitor of choline uptake, hemicholinium-3, or vesicular acetylcholine transport, vesamicol, abolished these actions of Hon-Chi. Also, physostigmine at concentration sufficient to inhibit acetylcholinesterase enhanced the actions of Hon-Chi. Mediation of ACh release from the nerve terminals to enhance insulin secretion by Hon-Chi can thus be considered. Both the plasma glucose lowering action and the raised plasma levels of insulin and C-peptide induced by Hon-Chi were also inhibited by 4-diphenylacetoxy-N-methylpiperdine methiodide (4-DAMP), but not affected by the ganglionic nicotinic antagonist, pentolinium or hexamethonium, indicating the mediation of muscarinic M-3 receptors. The results suggest that Hon-Chi has an ability to raise the release of ACh from nerve terminals, which in turn to stimulate muscarinic M-3 receptors in pancreatic cells and augment the insulin release to result in plasma glucose lowering action. Thus, Hon-Chi seems suitable to employ as the health food for increase of insulin secretion in the prevention of type-2 diabetes. (c) 2006 Elsevier Ireland Ltd. All rights reserved.