Intranasal immunization with a recombinant truncated FimH adhesin adjuvanted with CpG oligodeoxynucleotides protects mice against uropathogenic Escherichia coli challenge
Intranasal immunization with a recombinant truncated FimH adhesin adjuvanted with CpG oligodeoxynucleotides protects mice against uropathogenic Escherichia coli challenge
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DOI：
10.1139/w06-065
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发表时间：
2006-11-01
影响因子：
2.8
通讯作者：
Scodeller, Eduardo Alberto
中科院分区：
文献类型：
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作者：
Poggio, Thelma Veronica;La Torre, Jose Leonardo;Scodeller, Eduardo Alberto
在这项工作中，我们评估了一种实验性鼻腔疫苗对尿路感染的疗效。该疫苗含有重组截短FimH(RFimHt)粘附素和CpG寡核苷酸。将该疫苗的效力与肌肉注射疫苗进行了比较，后者是用相同的免疫原加弗氏佐剂配制的。我们的结果表明，在两种情况下，接种疫苗的动物的血清免疫球蛋白G效价都有类似的提高。然而，鼻腔注射疫苗比肌肉注射疫苗产生更高的阴道冲洗液特异性免疫球蛋白A效价。在小鼠膀胱炎模型中，这两种疫苗都将尿路致病性大肠杆菌在膀胱的体内定植减少了100多倍。我们的结果表明，重组截短FimH粘附素+CpG寡核苷酸是一种合适的免疫原性组合，有助于开发高效的尿路感染疫苗。
In this work, we assessed the efficacy of an experimental intranasal vaccine against urinary-tract infections. The vaccine contained a recombinant truncated FimH (rFimHt) adhesin plus CpG oligodeoxynucleotides. The efficacy of the vaccine was compared with that of an intramuscular vaccine that was formulated with the same immunogen plus Freund's adjuvant. Our results show that serum immunoglobulin G titers of vaccinated animals were similarly enhanced in both cases. However, the intranasal vaccine elicited higher vaginal-wash-specific immunoglobulin A titers against rFimHt than the intramuscular route. Both vaccines reduced the in vivo colonization of the bladder by uropathogenic Escherichia coli more than 100-fold in a murine cystitis model. Our results indicate that a recombinant truncated FimH adhesin plus CpG oligodeoxynucleotides is a suitable immunogenic combination that can contribute to the development of a highly efficacious urinary tract infection vaccine.