Magnetic nanoparticle clusters for photothermal therapy with near-infrared irradiation
Magnetic nanoparticle clusters for photothermal therapy with near-infrared irradiation
复制标题
用于近红外照射光热治疗的磁性纳米颗粒簇
DOI：
10.1016/j.biomaterials.2014.10.064
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发表时间：
2015-01-01
期刊：
影响因子：
14
通讯作者：
Yang, Wuli
中科院分区：
文献类型：
--
作者：
Shen, Shun;Wang, Sheng;Yang, Wuli
在这项研究中，磁性纳米颗粒团簇的光热效应首次被报道用于肿瘤光热消融的体外细胞系统和体内研究。与单个磁性Fe3O4纳米颗粒（NPs）相比，簇状Fe3O4纳米颗粒可以显著提高近红外（NIR）吸收。在808 nm近红外辐照下，诱导较高温度的Fe3O4 NPs簇比单个Fe3O4 NPs对A549细胞具有更强的细胞毒性。然后我们进行了体内光热疗法（PTT）研究，并观察到有希望的肿瘤治疗。与PBS和近红外辐照的单个磁性Fe3O4 NPs相比，集束Fe3O4 NPs治疗效果更高。并对不同近红外照射时间下簇状Fe3O4 NPs的处理效果进行了评价。结果表明，近红外激光产生的持续高温和较长的照射时间对肿瘤细胞的杀伤效果更好。进一步采用H&E染色组织学分析和TUNEL免疫组织学分析评价PIT对A549肿瘤的抗肿瘤疗效。(C) 2014 Elsevier Ltd.版权所有。
In this study, the photothermal effect of magnetic nanoparticle clusters was firstly reported for the photothermal ablation of tumors both in vitro in cellular systems but also in vivo study. Compared with individual magnetic Fe3O4 nanoparticles (NPs), clustered Fe3O4 NPs can result in a significant increase in the near-infrared (NIR) absorption. Upon NIR irradiation at 808 nm, clustered Fe3O4 NPs inducing higher temperature were more cytotoxic against A549 cells than individual Fe3O4 NPs. We then performed in vivo photothermal therapy (PTT) studies and observed a promising tumor treatment. Compared with PBS and individual magnetic Fe3O4 NPs by NIR irradiation, the clustered Fe3O4 NPs treatment showed a higher therapeutic efficacy. The treatment effects of clustered Fe3O4 NPs with different time of NIR illumination were also evaluated. The result indicated that a sustained high temperature generated by NIR laser with long irradiation time was more effective in killing tumor cells. Furthermore, histological analysis of H&E staining and TUNEL immunohistological assay were further employed for antitumor efficacy assessment of PIT against A549 tumors. (C) 2014 Elsevier Ltd. All rights reserved.