Molecular Cloning and Expression Analysis of Bat Toll-Like Receptors 3, 7 and 9
Molecular Cloning and Expression Analysis of Bat Toll-Like Receptors 3, 7 and 9
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DOI：
10.1292/jvms.09-0050
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发表时间：
2010-02-01
影响因子：
1.2
通讯作者：
Yoshikawa, Yasuhiro
中科院分区：
文献类型：
--
作者：
Iha, Koichiro;Omatsu, Tsutomu;Yoshikawa, Yasuhiro
本研究合成了Toll样受体（TLR）3、7和9的cDNA并进行了全序列测定。蝙蝠TLR3的cDNA编码区。TLR7和TLR9的长度分别为2，718、3，150和3，090 bp。开放阅读框分别编码TLR 3、TLR 7和TLR 9的905、1，049和1，029个氨基酸。3个蝙蝠类TLRs的核苷酸序列、预测的氨基酸序列和预测的结构域与其他哺乳动物类TLRs的核苷酸序列、预测的氨基酸序列和结构域具有较高的同源性。此外，分析了各TLR在主要器官中的表达谱。TLR3在肝脏中的表达最高，而TLR7和TLR9在脾脏中的表达最高。
In this study, cDNA of Toll-like receptors (TLR) 3, 7 and 9 were synthesized and completely sequenced. The coding regions of cDNA for bat TLR3. TLR7 and TLR9 were 2,718, 3,150 and 3,090 bp in length, respectively. The open reading frames encoded 905, 1,049 and 1,029 amino acids for TLR3, TLR7 and TLR9, respectively. The nucleotide sequences, predicted amino acid sequences and predicted domain structures of the three bat TLRs had high homology with those of other mammals. In addition, the expression profiles of each TLR in main organs were analyzed. Expression of TLR3 was highest in the liver, whereas the expressions of TLR7 and TLR9 were highest in the spleen.