Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment.
Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment.
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人肝型丙酮酸激酶：cDNA 克隆和染色体分配。
DOI：
10.1016/0006-291x(87)91372-6
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发表时间：
1987
影响因子：
3.1
通讯作者：
S. Miwa
中科院分区：
文献类型：
--
作者：
K. Tani;H. Fujii;H. Tsutsumi;J. Sukegawa;K. Toyoshima;M. Yoshida;T. Noguchi;T. Tanaka;S. Miwa
丙酮酸激酶（PK）有四种同工酶（L，R.M1,M2），主要由两个不同的基因编码。利用大鼠肝脏(L)型PK cDNA探针从日本成人肝脏λgt10 cDNA文库中分离到一个cDNA克隆。一个正杂交克隆hlPK-1，含有1049个碱基对的cDNA插入，进行DNA序列分析。序列数据与大鼠PK cDNA的比较表明，该cDNA编码了人类l型PK的羧基端105个氨基酸和3 '未翻译区734个核苷酸的信息。此外，对几种人鼠杂交细胞的核型分析和对含有hlPK-1的杂交细胞dna的Southern blot分析表明，人类l型PK基因位于1号染色体上。
Pyruvate kinase(PK) has four isozymes (L,R.M1,M2) that are encoded mainly by two different genes. We isolated a cDNA clone from a japanese adult liver λgt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA inset, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3′untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.
影响因子：
2.9
作者：
YOSHITAKE, S;SCHACH, BG;KURACHI, K
通讯作者：
KURACHI, K