Photoinitiator‐Free Two‐Photon Polymerization of Biocompatible Materials for 3D Micro/Nanofabrication
Photoinitiator‐Free Two‐Photon Polymerization of Biocompatible Materials for 3D Micro/Nanofabrication
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DOI：
10.1002/adom.202200474
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发表时间：
2022-07
影响因子：
9
通讯作者：
Atsushi Nakayama;Y. Kumamoto;Masafumi Minoshima;K. Kikuchi;A. Taguchi;K. Fujita
中科院分区：
文献类型：
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作者：
Atsushi Nakayama;Y. Kumamoto;Masafumi Minoshima;K. Kikuchi;A. Taguchi;K. Fujita
双光子聚合因其具有亚微米空间分辨率的3D制造能力而有望实现再生医学和药物开发生物组织的制造。然而，通常添加到树脂中的光聚合引发剂生物相容性和水溶性较低，这对光聚合制备的微纳米结构的生物应用提出了挑战。在这项研究中，使用不含光引发剂的生物相容性材料进行双光子聚合，并评估了所制造的 3D 结构的材料特性的保存情况。使用波长为 400 nm 的飞秒脉冲激光成功制备了聚乙二醇二丙烯酸酯 (PEGda) 和胶原蛋白，特征尺寸为 100 nm，无需光引发剂。 PEGda 和胶原蛋白聚合所需的激发强度分别约为 450 和 150 kW cm−2，这比使用光引发剂报道的激发强度要小。拉曼光谱用于确认 PEGda 的水膨胀聚合物网络和胶原蛋白的酶促降解（它们是活细胞应用中的重要因素）在制造的结构中得以保留。
Two‐photon polymerization is expected to realize the fabrication of biological tissues for regenerative medicine and drug development because of its capability of 3D fabrication with submicrometer spatial resolution. However, photopolymerization initiators generally added to resins have low biocompatibility and water solubility, which pose a challenge for the biological application of micro‐ and nanostructures fabricated by photopolymerization. In this study, two‐photon polymerization is performed using biocompatible materials without photoinitiators and the preservation of the material properties of the fabricated 3D structures is evaluated. Poly(ethylene glycol) diacrylate (PEGda) and collagen are successfully fabricated using a femtosecond pulsed laser at a wavelength of 400 nm with a feature size of 100 nm without photoinitiator. The excitation intensities required for the polymerization of PEGda and collagen are about 450 and 150 kW cm−2, respectively, which are smaller than those reported with using photoinitiators. Raman spectroscopy is used to confirm that the water‐swelling polymer network of PEGda and the enzymatic degradation of collagen, which are important factors in the application with live cells, are maintained in the fabricated structures.