Preferential overexpression of a class MU glutathione S-transferase subunit in mouse liver by myristicin
Preferential overexpression of a class MU glutathione S-transferase subunit in mouse liver by myristicin
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DOI：
10.1006/bbrc.1997.7053
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发表时间：
1997-07-30
影响因子：
3.1
通讯作者：
Tobola, AS
中科院分区：
文献类型：
--
作者：
Ahmad, H;Tijerina, MT;Tobola, AS
本研究旨在探讨欧芹叶活性成分肉豆蔻醚诱导小鼠肝脏谷胱甘肽S-转移酶（GST）的机制。A/J白化病小鼠，给予5至50毫克剂量的肉豆蔻醚，显示出4至14倍的增加，在肝脏GST比活性的控制，GST纯化等量的控制和肉豆蔻醚处理的肝脏GST活性显着增加。对2，4-二氯硝基苯的GST活性相对较高的增加和肉豆蔻酸处理的小鼠肝脏的Western印迹分析上GST μ水平的显著增加表明GST μ的优先诱导。研究结果还表明，在肝脏中表达的两种mu类GST亚基（Mr.26，500和Mr.25，000）中，只有一种（Mr.26，500）显著升高。肉豆蔻酸处理引起GST Pl水平的轻微变化，而GST α的水平显示出适度的增加。这些结果表明，肉豆蔻酸可能是一种有效的化学预防剂，特别是对于被mu类GST解毒的致癌物。(C)北京：科学出版社.
The present studies were undertaken to elucidate the mechanism of induction of glutathione S-transferase (GST) in mouse liver by myristicin, an active constituent of parsley leaf. A/J albino mice, given 5 to 50 mg doses of myristicin, showed 4-to 14-fold increase in liver GST specific activity over the control, GST purified from equal amounts of control and myristicin-treated livers indicated a marked increase in the GST activity. A relatively higher increase in GST activity towards 2,4-dichloronitrobenzene and a profound increase in the levels of GST mu on Western blot analysis of the myristicin-treated mouse liver suggest a preferential induction of GST mu. Results of the study also indicate that out of the two mu class GST subunits (Mr. 26,500 and Mr. 25,000) expressed in liver only one (Mr. 26,500) is significantly elevated. Myristicin treatment caused a slight change in the GST pi levels while the levels of GST alpha showed a modest increase. These results suggest that myristicin could be an effective chemopreventive agent, particularly for carcinogens that are detoxified by the mu class GST. (C) 1997 Academic Press.