Construction and immunogenicity of pseudotype baculovirus expressing GP5 and M protein of porcine reproductive and respiratory syndrome virus
Construction and immunogenicity of pseudotype baculovirus expressing GP5 and M protein of porcine reproductive and respiratory syndrome virus
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猪繁殖与呼吸综合征病毒GP5和M蛋白假型杆状病毒的构建及免疫原性
DOI：
10.1016/j.vaccine.2007.09.069
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发表时间：
2007-11-28
期刊：
影响因子：
5.5
通讯作者：
Xiao, Shaobo
中科院分区：
文献类型：
--
作者：
Wang, Shengping;Fang, Liurong;Xiao, Shaobo
杆状病毒是一种在昆虫细胞中广泛应用的生产重组蛋白的优良工具，最近成为一种新的、有吸引力的哺乳动物细胞基因传递载体。本研究以假杆状病毒（包膜上有水疱性口炎病毒（VSV-G）糖蛋白）为载体，在两种独立的巨细胞病毒即时早期（CMV-IE）增强子/启动子的转录调控下，构建了共表达猪繁殖与呼吸综合征病毒（PRRSV） GP5和M蛋白的重组杆状病毒。重组杆状病毒（BV-G-5m6）在哺乳动物细胞中高效表达PRRSV GP5和M蛋白。以不同剂量(1 × 10(8)、1 × 10（9)和1 × 10(10) PFU/小鼠）肌肉注射BV-G-5m6诱导prrsv特异性中和抗体和γ干扰素（ifn - γ）的产生，并呈剂量依赖性。此外，即使在低剂量（10(8)PFU）下，BV-G-5m6也比编码相同抗原的DNA构建体（pCI-5m6）具有更好的免疫原性，这可以通过显著增强中和抗体和ifn - γ的产生来证明。这些结果表明，假杆状病毒介导的基因传递可以作为开发新一代PRRSV感染疫苗的替代策略。(C) 2007 Elsevier Ltd.版权所有。
Baculovirus, which is extensively utilized as an excellent tool for production of recombinant protein in insect cells, has recently emerged as a novel and attractive gene delivery vehicle for mammalian cells. In the present study, a pseudotype baculovirus (with the glycoprotein of vesicular stomatitis virus (VSV-G) on the envelope) was used as vector to construct recombinant baculovirus coexpressing GP5 and M protein of porcine reproductive and respiratory syndrome virus (PRRSV), under the transcriptional control of two independent cytomegalovirus immediate early (CMV-IE) enhancer/promoters. The resultant recombinant baculovirus (BV-G-5m6) efficiently expressed PRRSV GP5 and M protein in mammalian cells. Intramuscular injection of BV-G-5m6 with various doses (1 x 10(8), 1 x 10(9), and 1 x 10(10) PFU/mouse) induced the production of PRRSV-specific neutralizing antibodies and gamma interferon (IFN-gamma) under dose-dependent pattern. Furthermore, BV-G-5m6 performed better immunogenicity, even at low dose (10(8) PFU), than DNA construct (pCI-5m6) encoding the same antigens, as demonstrated by significantly enhanced neutralizing antibodies and IFN-gamma production. These results indicate that pseudotype baculovirus-mediated gene delivery can be utilized as an alternative strategy to develop new generation of vaccine against PRRSV infection. (C) 2007 Elsevier Ltd. All rights reserved.