The antioxidant protein ZmPrx5 contributes resistance to maize stalk rot
The antioxidant protein ZmPrx5 contributes resistance to maize stalk rot
复制标题
抗氧化蛋白 ZmPrx5 有助于抵抗玉米茎腐病
DOI：
10.1016/j.cj.2021.12.001
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发表时间：
2022-08-01
期刊：
影响因子：
6.6
通讯作者：
Wu，Liuji
中科院分区：
文献类型：
--
作者：
Wang，Shunxi;Liu，Wencheng;Wu，Liuji
玉米（Zea maysL.）茎腐病是一种世界性的毁灭性病害，造成严重的产量损失。尽管以往的研究主要集中在玉米对茎腐病抗性的遗传分析上，但其抗性机制仍不清楚。我们使用比较蛋白质组学方法来鉴定与茎腐病抗性相关的候选蛋白。统计分析表明，禾谷镰刀菌和模拟接种植物之间差异积累的蛋白质有763个。其中，ZmPrx 5在病害植株中大量表达，ZmPrx 5在根、茎、叶、穗和生殖器官中均有表达。ZmPrx 5基因在3个自交系中的表达量在F.禾谷镰刀菌感染ZmPrx 5定位于细胞质中。与对照相比，ZmPrx 5过表达的玉米植株对F. ZmPrx 5突变体植株比野生型植株更易感染禾谷镰刀菌。与野生型植物相比，ZmPrx 5纯合突变体植物中的防御相关途径，包括植物-病原体相互作用、苯丙氨酸代谢、苯并恶嗪类化合物和类黄酮生物合成受到抑制。我们认为，ZmPrx 5积极调节玉米茎腐病的抗性，可能是通过防御导向的转录组重编程。这些结果为进一步研究Prx 5亚家族蛋白在植物抗真菌病害中的作用奠定了基础，也为玉米抗病育种提供了潜在的遗传资源。
Maize (Zea maysL.) stalk rot is a devastating disease worldwide, causing severe yield losses. Although previous studies have focused on the genetic dissection of maize resistance to stalk rot, the mechanisms of resistance remain largely unknown. We used a comparative proteomics approach to identify candidate proteins associated with stalk rot resistance. Statistical analyses revealed 763 proteins differentially accumulated betweenFusarium graminearumand mock-inoculated plants. Among them, the antioxidant protein ZmPrx5, which was up-accumulated in diseased plants, was selected for further study.ZmPrx5transcripts were present in root, stalk, leaf, ear, and reproductive tissues. The expression ofZmPrx5in three inbred lines increased significantly uponF. graminearuminfection. ZmPrx5 was localized in the cytoplasm. Compared to control plants, maize plants overexpressingZmPrx5showed increased resistance toF. graminearuminfection, andZmPrx5mutant plants were more susceptible than wild-type plants. Defense-associated pathways including plant–pathogen interactions, phenylalanine metabolism, and benzoxazinoid and flavonoid biosynthesis were suppressed inZmPrx5homozygous mutant plants compared with wild-type plants. We suggest that ZmPrx5 positively regulates resistance against stalk rot in maize, likely through defense-oriented transcriptome reprogramming. These results lay a foundation for further research on the roles of Prx5 subfamily proteins in resistance to plant fungal diseases, and provide a potential genetic resource for breeding disease-resistance maize lines.