Antigenic and genetic relationships between European very virulent infectious bursal disease viruses and an early West African isolate
Antigenic and genetic relationships between European very virulent infectious bursal disease viruses and an early West African isolate
复制标题
DOI：
10.1080/03079459995028
复制
发表时间：
1999-02-01
期刊：
影响因子：
2.8
通讯作者：
Skinner, MA
中科院分区：
文献类型：
--
作者：
Eterradossi, N;Arnauld, C;Skinner, MA
研究了不同国家传染性法氏囊病病毒（IBDV）的抗原性和遗传关系。使用基于一组7种中和单克隆抗体（Mab）的抗原捕获ELISA进行抗原表征，所述中和单克隆抗体（Mab）探测至少3个位于VP 2的抗原结构域。所有这些结构域在Faragher 52/70（F52/70）参考株中对欧洲经典血清型1 IBDV具有反应性。通过逆转录、扩增和编码VP 2可变结构域的基因组片段的直接测序来实现基因组表征。将11株法国vv分离株与英国、荷兰和比利时的UK 661、DV 86和849 VB病毒以及1988年从象牙海岸获得的早期vv分离株进行了比较。所有病毒均表现出不结合Mab 3和4的抗原谱。Mab 3和4的缺乏结合可能有助于区分经典和vvIBDV。没有一个非法国毒株类似于91168和94432法国分离株，它们不结合Mab 6、7或8。遗传分析显示，所有欧洲病毒之间关系密切，彼此之间的差异不超过12个核苷酸和3个氨基酸。非洲分离株与所有欧洲病毒（包括F52/70病毒）明显不同，至少有22个核苷酸和6个氨基酸差异。基于相邻连接和简约方法的系统发育分析表明，非洲病毒可能属于高致病性IBDV的遗传上不同的谱系。
The antigenic and genetic relationships between very virulent (vv) infectious bursal disease viruses (IBDV) from different countries were investigated. Antigenic characterization was performed using an antigen-capture ELISA based on a panel of seven neutralizing monoclonal antibodies (Mabs), which probe at least three VP2-located antigenic domains. All these domains are reactive in the Faragher 52/70 (F52/70) reference strain for European classical serotype 1 IBDV, Genomic characterization was achieved by reverse transcription, amplification and direct sequencing of a genome fragment encoding the VP2 variable domain. Eleven vv isolates from France were compared to the British, Dutch and Belgian UK661, DV86 and 849VB viruses, and to an early vv isolate obtained from the Ivory Coast in 1988, All viruses exhibited antigenic profiles characterized by no binding of Mabs 3 and 4, Lack of binding of Mabs 3 and 4 might thus be helpful for differentiating classical and vvIBDVs, None of the non-French strains resembled the 91168 and 94432 French isolates, which did not bind Mabs 6, 7 or 8, The genetic analysis revealed close relationships between all the European viruses, which differed from one another by no more than 12 nucleotides and 3 amino acids. The African isolate was markedly different, with at least 22 nucleotide and six amino acid differences to all the European viruses, including the F52/70 virus. Phylogenetic analysis based on the neighbour-joining and parsimony methods suggest that the African virus may belong to a genetically distinct lineage of highly pathogenic IBDVs.